| 双波长紫外协同强化消毒及对光、暗复活影响 |
| 摘要点击 958 全文点击 183 投稿时间：2024-01-17 修订日期：2024-05-10 |
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| 中文关键词 紫外线 消毒 双波长 协同作用 微生物复活 |
| 英文关键词 ultraviolet disinfection dual-wavelength synergistic effect bacterial reactivation |
| 作者 | 单位 | E-mail | | 周添红 | 兰州交通大学环境与市政工程学院, 兰州 730070 | zhouth@163.com | | 梁源夫 | 兰州交通大学环境与市政工程学院, 兰州 730070
中国科学院生态环境研究中心环境水质学国家重点实验室, 北京 100085 | 1317451163@qq.com | | 孙喆 | 中国科学院生态环境研究中心环境水质学国家重点实验室, 北京 100085 | | | 李梦凯 | 中国科学院生态环境研究中心环境水质学国家重点实验室, 北京 100085 | | | 王佳乐 | 中国科学院生态环境研究中心环境水质学国家重点实验室, 北京 100085 | | | 李文涛 | 中国科学院生态环境研究中心环境水质学国家重点实验室, 北京 100085 | | | 贲伟伟 | 中国科学院生态环境研究中心环境水质学国家重点实验室, 北京 100085 | | | 张国珍 | 兰州交通大学环境与市政工程学院, 兰州 730070 | | | 强志民 | 中国科学院生态环境研究中心环境水质学国家重点实验室, 北京 100085 | |
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| 中文摘要 |
| C波段紫外线（UV-C）可有效灭活水中病原微生物,远UV-C（200~230 nm）和常规UV-C（250~280 nm）可分别损伤病原微生物的蛋白质和核酸,同时辐照有望实现病原微生物的协同灭活. 然而由于现有实验装置难以满足研究需求,相关研究十分有限. 因此,搭建了配装KrCl准分子灯和低压汞灯的细管流光反应系统,可单独/同时输出稳定的222 nm（远UV-C）和254 nm（常规UV-C）辐照. 之后利用该装置探究了双波长UV-C对大肠杆菌（E. coli）的协同灭活作用,以及对光、暗复活的影响. 结果表明,双波长UV-C协同灭活E. coli作用显著,协同系数最高可达2.2. 此外,双波长UV-C辐照明显减弱E. coli的光复活作用,最大对数复活率从254 nm单独辐照的50.8%降低为36.1%,同时,在黑暗条件下,未发现复活现象且呈现暗凋零现象,说明双波长UV-C辐照可降低光、暗复活风险,确保水质生物安全,研究可为高效和安全的水消毒技术研发提供理论依据. |
| 英文摘要 |
| Ultraviolet-C （UV-C） irradiation can effectively deactivate pathogenic microorganisms. The far UV-C （200-230 nm） and conventional UV-C （250-280 nm） can damage the proteins and nucleic acids of pathogenic microorganisms, respectively. The combination of far and conventional UV-C has the potential of synergistic inactivation. However, relevant studies remain limited owing to the lack of appropriate experimental setups. Therefore, this study established a mini-fluidic photoreaction system equipped with a KrCl excimer lamp and a low-pressure mercury lamp. This system could independently/simultaneously deliver stable 222 nm （far UV-C） and 254 nm （conventional UV-C） irradiations. Subsequently, the system was used to investigate the synergistic effect of dual-wavelength UV-C （the combination of 222 nm and 254 nm UV-C） on Escherichia coli （E. coli） inactivation and the subsequent impact on photo- and dark-reactivation. The results indicated that dual-wavelength UV-C had a significant synergistic effect on E. coli inactivation, with a synergistic coefficient up to 2.2. Additionally, comparing to 254 nm UV irradiation, the photo-reactivation of E. coli after dual-wavelength UV-C irradiation was weakened, with the maximum lg reactivation percentage reducing from 50.8% to 36.1%. Furthermore, E. coli after dual-wavelength UV-C irradiation exhibited dark decay, in which the inactivation efficiency was further enhanced during dark treatment. Therefore, these results suggest that dual-wavelength UV-C could inhibit the light reactivation and dark repair of E. coli, ensuring the water biosafety, as well as providing reference for the development of effective and safe water disinfection technologies. |
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