| 生物降解地膜农田填埋过程中膜表面细菌群落演替过程 |
| 摘要点击 898 全文点击 177 投稿时间：2024-02-04 修订日期：2024-04-19 |
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| 中文关键词 生物降解地膜（BDM） 细菌群落多样性 群落组成 群落功能 网络分析 |
| 英文关键词 biodegradable film（BDM） bacterial diversity community composition bacterial function network analysis |
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| 中文摘要 |
| 随着农业现代化的快速发展,生物降解地膜在农田中的使用越来越广泛. 然而,关于生物降解地膜对土壤细菌群落的影响仍知之甚少. 研究选用生物降解地膜（PBAT+PLA）在农田中进行了为期75 d的填埋实验,采用Illumina NovaSeq高通量测序技术,对不同填埋时期的生物降解地膜表面的细菌群落进行分析. 结果表明,生物降解地膜（H8）表面的细菌α多样性显著小于对照土壤（CK）. 此外,与填埋初期相比,填埋中后期H8处理中细菌α多样性显著下降（P<0.05）. CK和H8处理中细菌群落结构显著不同（P<0.01）. H8处理中的变形菌门（Proteobacteria）相对丰度显著大于CK处理（P<0.05）. LEfSe分析发现,和CK处理相比,在填埋初期H8处理中差异显著的类群为假节杆菌属（Pseudarthrobacter）和食酸菌属（Acidovorax）,填埋中期差异显著的类群为贪噬菌属（Variovorax）和分支杆菌属（Mycobacterium）,填埋后期差异显著的类群为噬氢菌属（Hydrogenophaga）和黄色金线菌属（Chryseolinea）,以上类群可以被看做是该阶段的指示物种. 基于FAPROTAX对细菌群落功能进行分析发现,在填埋处理的初期和中期,H8处理中细菌群落与碳代谢相关的功能得到了增强. 网络分析发现,与CK处理相比,H8处理中的细菌群落拥有较简单的网络结构和较高的模块化值以及更多的负相关连接数. 研究结果可为深入了解生物降解地膜对土壤微生态环境影响提供科学依据. |
| 英文摘要 |
| With the rapid advancement of agricultural modernization, biodegradable mulch （BDM） in farmland has become increasingly prevalent. However, little is known about the effects of BDM buried in soil on bacterial communities. In this study, a burial experiment was conducted on farmland using BDM （PBAT+PLA）. The bacterial communities on the surface of the BDM （H8） at different burial stages were analyzed through Illumina NovaSeq high-throughput sequencing technology. The landfill experiment was carried out for a total of 75 days. The results revealed that the bacterial α-diversity in BDM was significantly lower than that in the control soil, with a notable decrease in soil bacterial α-diversity on the BDM surface in the later stages of burial compared to that in the early stages （P<0.05）. The bacterial community of control soil was significantly different from that of BDM （P<0.01）. The relative abundance of the Proteobacteria phylum in the BDM was significantly higher than that in the control soil （P<0.05）. LEfSe analysis revealed significant differences in taxa between the buried BDM and the control group across burial stages. During the early stage, Pseudarthrobacter and Acidovorax showed significant differences. Variovorax and Mycobacterium exhibited differences in the mid-stage, whereas Hydrogenophaga and Chryseolinea differed significantly in the late stage. These taxa served as indicator species for their corresponding burial stages. Functional analysis of bacterial communities revealed an enhancement of carbon metabolism-related functions in the bacterial community on the BDM surface during the early and middle stages. Moreover, network analysis revealed that, compared to that in the control soil, BDM exhibited a simpler network structure of the bacterial community, higher modularity values, and more negative correlation connections. These findings collectively provide a scientific foundation for a comprehensive understanding of the impact of BDM on the soil microecological environment. |
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