| 冀北采石场恢复区不同植被恢复类型对土壤微生物群落结构的影响 |
| 摘要点击 1111 全文点击 152 投稿时间：2024-02-08 修订日期：2024-04-21 |
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| 中文关键词 土壤微生物 土壤理化性质 植被恢复类型 采石场 冀北 |
| 英文关键词 soil microorganisms soil physicochemical properties vegetation restoration types quarries northern Hebei Province |
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| 中文摘要 |
| 土壤微生物对矿区土壤环境及植被恢复效益有很强的影响作用,为探究中国冀北地区采石场土壤微生物多样性对不同植被恢复类型的响应,以该区常见恢复用植被[油松（YS）、沙棘（SJ）、苜蓿（MX）、棉槐（MH）和刺槐（CH） ]为研究对象,利用土壤养分测定技术、高通量测序技术等方法对植被根际土壤理化因子及微生物群落结构进行分析,结论表明：①植被恢复类型对根际土壤环境影响显著,沙棘和油松根际土壤碱解氮含量、棉槐土壤有机质含量以及苜蓿、棉槐、刺槐中的微生物碳含量均显著高于对照组（P<0.05）. ②不同植被类型中细菌和真菌的群落组成和多样性差异明显. 变形菌门（Proteobacteria）和子囊菌门（Ascomycota）分别为细菌和真菌的主要优势种群；油松和沙棘中拟杆菌门的相对丰度高于其他植物,苜蓿和刺槐的ACE和Chao1指数差异显著（P<0.05）,不同处理组的组间差异明显（stress<0.1）. ③不同恢复类型的微生物群落与土壤因子有显著相关性,细菌和真菌群落结构与SOM、TN和MBC等均呈现出极显著相关性（P<0.01）. 细菌菌群中,酸杆菌门（ Acidobacteriota）与微生物量氮含量和碱解氮含量等成正比；真菌群落中,油壶菌门（Olpidiomycota）与TK和pH成正相关,养分因子的单独作用在细菌门水平群落中最大,养分因子和酶活性因子的协同作用在真菌门水平群落中最大. 研究结论对后续矿区生态修复工作有理论指导意义. |
| 英文摘要 |
| Soil microorganisms have a strong influence on the soil environment of mining sites and the effectiveness of vegetation restoration. To investigate the response of soil microbial diversity in quarries in northern Jibei, China, to different types of vegetation restoration, we considered the common restoration vegetation in the area （YS, SJ, MX, MH, and CH） as the object of study and analyzed inter-root soil physicochemical factors and vegetation microbial community structure using the techniques of soil nutrient determination, high-throughput sequencing, and other methods. The results showed that： ① The type of vegetation restoration had a significant effect on the inter-root soil environment, and the alkaline dissolved nitrogen content of the inter-root soil of sea buckthorn and oil pine； the organic matter content of the soil of cotton acacia； and the microbial carbon content of lucerne, cotton acacia, and acacia were significantly higher than that of the control group （P<0.05）. ② The community composition and diversity of bacteria and fungi differed significantly among the different vegetation types. Proteobacteria and Ascomycota were the main dominant populations of bacteria and fungi, respectively. The relative abundance of Anabaena was higher than that of other plants in oil pine and sea buckthorn, and the ACE and Chao1 indices of lucerne and acacia differed significantly （P<0.05）, and the intergroup differences between different treatment groups were obvious （stress<0.1）. ③ The microbial communities of different restoration types were significantly correlated with soil factors, and the structure of bacterial and fungal communities showed highly significant correlations with SOM, TN, and MBC （P<0.01）. Among the bacterial communities, Acidobacteriota was positively correlated with microbial nitrogen content, alkaline dissolved nitrogen content, etc. Among the fungal communities； Olpidiomycota was positively correlated with TK and pH； the individual effects of nutrient factors were greatest in the bacterial phylum-level communities, and the synergistic effects of nutrient factors and enzyme activity factors were greatest in the fungal phylum-level communities. The conclusions of the study have theoretical implications for subsequent ecological restoration work in the mining area. |
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