| 污水处理过程逸散的生物气溶胶抗生素抗性分析 |
| 摘要点击 1556 全文点击 310 投稿时间：2023-09-01 修订日期：2023-11-01 |
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| 中文关键词 污水处理厂 生物气溶胶 抗生素抗性基因（ARGs） 抗生素抗性致病菌（PAPB） 来源 |
| 英文关键词 wastewater treatment plant bioaerosol antibiotic resistant genes（ARGs） pathogenic antibiotic resistant bacteria（PAPB） source |
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| 中文摘要 |
| 为探究污水处理厂生物气溶胶负载抗生素抗性基因（ARGs）和抗生素抗性致病菌（PARB）的赋存及来源.利用宏基因组测序与组装技术对山东省某污水处理厂生物气溶胶及污水样本的抗生素抗性基因组进行检测.结果表明,相比于上风向,污水处理厂和下风向生物气溶胶中具有更多的ARGs亚型种类数和更高丰度的PARB.污水处理厂生物气溶胶中主导的ARGs主型和亚型分别为多药类ARGs和macB. 37种PARB携带至少两种及以上的ARGs主型,表现出多重耐药性.对于细格栅、好氧池和污泥脱水间生物气溶胶样本,污水是ARGs和PARB最主要的来源.共检测到铜绿假单胞菌（Pseudomonas aeruginosa）和大肠埃希氏菌（Escherichia coli）等32种PARB在至少1个污水处理单元极易气溶胶化.研究将为污水处理厂生物气溶胶抗生素抗性污染风险评估及健康保障提供理论支持. |
| 英文摘要 |
| To explore the prevalence and source of antibiotic resistant genes （ARGs） and pathogenic antibiotic resistant bacteria （PARB） associated with bioaerosols in wastewater treatment plants （WWTPs）, metagenomic sequencing and assembly were applied to elucidate the antibiotic resistome of bioaerosols and wastewater in WWTPs. The results showed that more subtypes of ARGs and a higher abundance of PARB were found in bioaerosols from WWTPs and downwind than those from upwind. Multidrug and macB were respectively the most dominant type and subtype of ARGs in bioaerosols from WWTPs. In total, 37 types of PARB carried at least two or more ARG types and were characterized by multiple drug resistance. At the fine grid, aerated tank, and sludge dewatering room, wastewater was the main source of bioaerosol ARGs and PARB. A total of 32 PARB were easily aerosolized in at least one wastewater treatment unit, such as Pseudomonas aeruginosa and Escherichia coli. This study will provide theoretical support for the risk assessment and health protection of antibiotic resistant pollution associated with bioaerosols from WWTPs. |
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