| 模拟氮沉降对三江平原小叶章湿地土壤微生物碳源利用能力的影响 |
| 摘要点击 3099 全文点击 892 投稿时间：2021-11-28 修订日期：2022-01-29 |
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| 中文关键词 小叶章湿地 土壤微生物 土壤理化 碳源利用能力 Biolog-Eco微平板技术 |
| 英文关键词 Calamagrostis angustifolia wetland soil microorganisms soil physico-chemistry properties carbon source utilization capacity Biolog-Eco microplate technology |
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| 中文摘要 |
| 大气氮沉降会对湿地土壤微生物的结构和功能产生显著影响，因此研究不同浓度氮沉降对三江平原小叶章湿地土壤微生物碳源利用能力的影响，对于保护和利用湿地生态系统具有重要意义.采用Biolog-Eco技术探究了连续5 a模拟氮沉降对小叶章湿地土壤微生物碳源利用能力的影响.结果表明：①长期的氮素施加会显著改变小叶章湿地土壤含水量（SMC）、pH、硝态氮（NO3-）、铵态氮（NH4+）、溶解性有机碳（DOC）和总氮（TN）的含量（P<0.05）；②土壤微生物的平均颜色变化率（AWCD）呈现的规律为：对照（CK）>高氮（HN）>低氮（LN）.低氮处理显著降低了土壤微生物香农（Shannon）指数（P<0.05），高氮处理显著降低了土壤微生物皮卢（Pielou）指数（P<0.05）.③不同氮浓度下土壤微生物碳源利用能力显著不同，其中低氮处理显著抑制了土壤微生物对糖类、醇类、胺类和酸类的利用强度（P<0.05）；高氮处理显著促进了微生物对酯类的利用，但高氮使土壤微生物对糖类、胺类和酸类碳源产生抑制作用（P<0.05）.④冗余分析显示，NH4+、DOC和pH是影响三江平原小叶章湿地土壤微生物碳代谢能力的主要环境因子.长期氮沉降会导致土壤微生物功能多样性的降低，有关碳源底物利用的微生物活性也明显降低，并且微生物对单一碳源底物的利用能力也发生改变. |
| 英文摘要 |
| Atmospheric nitrogen deposition has a crucial impact on the structure and function of soil microorganisms of wetland ecosystems. Therefore, carrying out a study on the effects of soil carbon metabolism capacity has a great significance for the protection and utilization of wetland ecosystems. In this study, the effects of simulated nitrogen deposition on the carbon metabolic capacity of soil microorganisms in Calamagrostis angustifolia wetland for five consecutive years was investigated using Biolog-Eco technology. The results showed:① soil water content (SMC), pH, nitrate nitrogen (NO3-), ammonium nitrogen (NH4+), dissolved organic carbon (DOC), and total nitrogen (TN) contents were significantly different (P<0.05) under different nitrogen deposition conditions. ② The average well color development (AWCD) values of soil microorganisms within different N depositions were in the order of CK (control)>HN (high nitrogen treatment)>LN (low nitrogen treatment). LN significantly reduced the Shannon diversity index of soil microorganisms, and HN significantly reduced the Pielou index of soil microorganisms (P<0.05). ③ LN significantly inhibited the intensity of the utilization of carbohydrates, alcohols, amines, and acids by soil microorganisms (P<0.05); HN significantly promoted the utilization of esters by microorganisms, but HN caused soil microorganisms to inhibit the carbon sources of carbohydrates, amines, and acids (P<0.05). ④ Redundancy analysis showed that NH4+, DOC, and pH were the main environmental factors affecting the functional diversity of soil microbial communities in Calamagrostis angustifolia wetland in the Sanjiang Plain. Long-term nitrogen deposition will lead to the reduction in soil microbial functional diversity; the microbial activity related to the utilization of carbon source substrates is also significantly reduced, and the ability of microorganisms to utilize a single carbon source substrate also changes. |
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