| 废水排放对近海环境中抗生素抗性基因和微生物群落的影响 |
| 摘要点击 1680 全文点击 552 投稿时间：2021-12-27 修订日期：2022-01-17 |
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| 中文关键词 近海环境 废水排放 抗生素抗性基因(ARGs) 可移动遗传元件(MGEs) 微生物群落 |
| 英文关键词 coastal area wastewater discharge antibiotic resistance genes (ARGs) mobile genetic elements (MGEs) microbial community |
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| 中文摘要 |
| 污水处理厂是水环境中抗生素抗性基因（ARGs）的重要来源.可移动遗传元件（MGEs）和微生物群落是影响ARGs增殖扩散的关键因素.为探究污水处理厂废水排放对近海环境中ARGs和微生物群落的影响，采用高通量荧光定量PCR （HT-qPCR）和高通量16S rRNA扩增子测序技术，对杭州湾上虞（SY）和嘉兴（JX）两个近岸纳污区（ERAs）及远岸湾区表层沉积物中ARGs、MGEs和微生物群落的组成和分布进行调查.结果表明，多重耐药类ARGs是所有样点中丰度最高的ARGs类型.纳污区沉积物中ARGs和MGEs多样性和丰度远远高于远岸湾区沉积物.JX纳污区沉积物中微生物群落丰富度和多样性高于SY纳污区及远岸湾区沉积物.PCoA结果显示，纳污区与远岸湾区沉积物中ARGs、MGEs和微生物群落分布存在显著的差异，说明长期的废水排放对近海环境中ARGs、MGEs和微生物群落影响较大.ARGs、MGEs和细菌属的共现网络显示，嗜冷杆菌属、假单胞菌属、亚硫酸杆菌属、假交替单胞菌属和芽孢杆菌属等12种菌属与ARGs和MGEs存在显著正相关.多重耐药类和β-内酰胺类ARGs的潜在宿主最多. |
| 英文摘要 |
| Wastewater treatment plants (WWTPs) are important sources of antibiotic resistance genes (ARGs) in aquatic environments. Mobile genetic elements (MGEs) and microbial communities are key factors that affect the proliferation of ARGs. To reveal the effects of WWTPs effluent discharge on the ARGs and microbial community in a coastal area, the structure and distribution of ARGs, MGEs, and microbial community in Shangyu (SY) and Jiaxing (JX) effluent receiving areas (ERAs) and the offshore area of Hangzhou Bay (HB) were investigated via high-throughput quantitative PCR and 16S rRNA high-throughput sequencing. The results showed that multidrug resistance genes were the most abundant ARGs across all the sampling sites. The diversity and abundance of ARGs and MGEs in the ERAs were much higher than those in the HB. Additionally, the diversities of the microbial community in the JX-ERA were higher than those in the SY-ERA and HB. PCoA showed that the distribution of ARGs, MGEs, and microbial communities in the ERAs and HB were significantly different, indicating that the long-term wastewater discharge could alter the distribution of ARGs, MGEs, and microbial communities in the coastal area. The co-occurrence pattern among ARGs, MGEs, and microbial communities revealed that 12 bacterial genera, such as Psychrobacter, Pseudomonas, Sulfitobacter, Pseudoalteromonas, and Bacillus, showed strong positive correlations with ARGs and MGEs. Most potential hosts carried multidrug and β-lactamase resistance genes. |
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