| 北运河京津冀段河道浮游微生物群落多样性变化分析 |
| 摘要点击 2085 全文点击 678 投稿时间：2021-06-01 修订日期：2021-07-27 |
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| 中文关键词 浮游微生物 空间变化 群落组成 多样性 环境解释 |
| 英文关键词 planktonic microorganisms spatial variability community composition diversity environmental interpretation |
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| 中文摘要 |
| 再生水在缓解城市水资源短缺方面发挥重要作用，但再生水中痕量污染物质与病原体对受纳水体中浮游微生物群落产生影响.基于OTUs和门水平的片段数量和片段丰度的浮游微生物数据矩阵探究北运河京津冀河道微生物群落多样性空间变化机制，结果表明，再生水的频繁汇入引起的物理和化学扰动改变了水体的水文和水质，使浮游微生物群落沿地理尺度分为中上游（MUC）和下游（DC）两个不同的类群.基于OTUs数据矩阵的多样性指数的分析显示DC类群物种多样性显著高于MUC类群，丰度分布及均匀度则呈现相反的趋势；物种丰富度主要由偶见菌群的片段多样性决定，而均匀度则主要由优势菌群的丰度变化决定；不同丰度等级的亚群落结构对空间变化的敏感性依次为：非优势菌群 > 偶见菌群 > 优势菌群.基于门水平的数据矩阵的多样性分析同样显示DC类群物种多样性显著高于MUC类群，丰度变化趋势相反；敏感微生物类群为非优势菌门，偶见菌门次之，优势菌门最不敏感；基于门水平片段数量的数据矩阵对环境变化的响应比基于门水平的丰度数据矩阵的响应更加敏感.与微生物类群显著相关的环境因子为排污口尺度的浊度（Turb）、高锰酸盐指数、氧化还原电位（ORP）、大环内酯类抗生素（MLs）、四环素类抗生素（TCs）响应因子和盐类离子、碳素、无机氮物质等区域尺度的响应因子两大类，其中，丰度和多样性方面，DC类群显著高于MUC类群的菌门与MLs显著负相关，而与TCs正相关显著；MUC类群显著高于DC类群的菌门则与MLs显著正相关.研究成果可为以再生水为主要补给水源的城市河道生态修复提供理论依据和技术指导. |
| 英文摘要 |
| Reclaimed water plays an important role in alleviating the shortage of urban water resources; however, the trace pollutants and pathogens in reclaimed water have an effect on the plankton community in the receiving water. This study investigated the spatial variation mechanism of microbial community diversity in the Beijing-Tianjin-Hebei reach of the Nordkanal River based on the OTUs and phylum level fragment number and fragment abundance data matrix. The results showed that the physical and chemical disturbance caused by the frequent inflow of reclaimed water changed the hydrology and water quality of the water body, and the plankton community could be divided into two different groups along the geographical scale:the medium and upstream clustering (MUC) and the downstream clustering (DC). The analysis of diversity index based on the OTUs data matrix showed that the species diversity of the DC group was significantly higher than that of the MUC group, and the abundance distribution and evenness showed the opposite trend. The species richness was mainly determined by the fragment diversity of the occasional microflora; the evenness was mainly determined by the variation of the abundance of the dominant microflora; the sensitivity of the subcommunity structure with different abundance levels to spatial change was in the order of non-dominant microflora > occasional microflora > dominant microflora. The diversity analysis of the data matrix based on phylum level also showed that the species diversity of the DC group was significantly higher than that of the MUC group, and the change trend of abundance was the opposite; the most sensitive microflora group was the non-dominant phyla, followed by the occasional phyla, and the dominant phyla group was the least sensitive. The data matrix based on the number of level segments of the gate was more sensitive to environmental changes than the multi-degree data matrix based on the level of the gate. The environmental factors significantly related to microbial community were turbidity; permanganate index; oxidation-reduction potential (ORP); macrolide (MLs); tetracycline antibiotic (TCs); and regional response factors of salt ions, carbon, and inorganic nitrogen. In the aspect of abundance and diversity, these phylas that the DC group was significantly more than the MUC group were more significantly negatively correlated with MLs, whereas they were positively correlated with TCs, and these phylas that the MUC group was significantly more than the DC group was more significantly positively correlated with MLs. The research results can provide a theoretical basis and technical guidance for the ecological rehabilitation of urban river courses with reclaimed water as their main water supply source. |
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