| 填料对ANAMMOX污泥活性恢复的影响及菌群特征 |
| 摘要点击 1898 全文点击 564 投稿时间：2021-05-26 修订日期：2021-06-15 |
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| 中文关键词 厌氧氨氧化(ANAMMOX) 填料 室温储存 活性恢复 菌群特征 |
| 英文关键词 anaerobic ammonium oxidizing (ANAMMOX) carrier room temperature storage activity recovery flora characteristics |
| 作者 | 单位 | E-mail | | 罗景文 | 中国环境科学研究院环境基准与风险评估国家重点实验室, 北京 100012
中国环境科学研究院国家环境保护地下水污染模拟与控制重点实验室, 北京 100012 | 13971650013@163.com | | 杨津津 | 中国环境科学研究院环境基准与风险评估国家重点实验室, 北京 100012
中国环境科学研究院国家环境保护地下水污染模拟与控制重点实验室, 北京 100012 | | | 李绍康 | 中国环境科学研究院环境基准与风险评估国家重点实验室, 北京 100012
中国环境科学研究院国家环境保护地下水污染模拟与控制重点实验室, 北京 100012 | | | 赵昕宇 | 中国环境科学研究院环境基准与风险评估国家重点实验室, 北京 100012
中国环境科学研究院国家环境保护地下水污染模拟与控制重点实验室, 北京 100012 | | | 杨一飞 | 中国环境科学研究院环境基准与风险评估国家重点实验室, 北京 100012
中国环境科学研究院国家环境保护地下水污染模拟与控制重点实验室, 北京 100012 | | | 韩嘉琛 | 中国环境科学研究院环境基准与风险评估国家重点实验室, 北京 100012
中国环境科学研究院国家环境保护地下水污染模拟与控制重点实验室, 北京 100012 | | | 李翔 | 中国环境科学研究院环境基准与风险评估国家重点实验室, 北京 100012
中国环境科学研究院国家环境保护地下水污染模拟与控制重点实验室, 北京 100012 | lixiang@craes.org.cn |
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| 中文摘要 |
| 为实现室温下长期储存的厌氧氨氧化污泥的活性快速恢复，采用3组反应器分别对在室温（15~30℃）且无基质的条件下储存9个月的厌氧氨氧化污泥进行活性恢复，其中R2和R3反应器分别添加彗星纤维填料和K3填料作为生物载体，探究不同填料添加对厌氧氨氧化污泥活性恢复速率的影响.结果表明，R2和R3反应器分别在第8 d和第10 d开始出现厌氧氨氧化反应，TIN去除率分别达82.25%和80.92%，明显优于未添加填料的R1反应器（第15 d开始出现，TIN去除率80.26%）.经过42 d的运行，进水ρ（NH4+-N）和ρ（NO2--N）分别达300 mg·L-1和396 mg·L-1，3组反应器的TIN去除率分别为78.96%、84.92%和84.66%.微生物群落结构分析显示，R2和R3反应器中厌氧氨氧化菌的相对丰度分别为6.85%和6.06%，是R1反应器的2~4倍，污泥中主要的厌氧氨氧化菌属为Candidatus Jettenia，其在3组反应器中的相对丰度分别为1.62%、5.74%和5.21%.综上，投加填料构建厌氧氨氧化生物膜-颗粒污泥复合系统，可以显著缩短在室温且无基质条件下长期储存的厌氧氨氧化污泥的活性恢复时间，填料有效促进了反应器中厌氧氨氧化菌相对丰度的提高，且彗星纤维填料的促进作用略优于K3填料. |
| 英文摘要 |
| In order to realize the rapid recovery of ANAMMOX sludge bacterial activity after long-term room temperature storage, three groups of reactors were added to ANAMMOX sludge that had been stored without substrate at room temperature (15-30℃) for 9 months. Among the three groups of reactors, comet fiber carrier and K3 carrier were added to R2 and R3 reactors, respectively, as biological carriers. The effects of different carriers on the recovery rate of ANAMMOX sludge bacterial activity were investigated. The results showed that ANAMMOX reactions in the R2 and R3 reactors began taking place on the 8th and 10th day, respectively, with respective TIN removal rates of 82.25% and 80.92%, which were significantly improved compared with that in the R1 reactor, in which no carrier was added (ANAMMOX reaction started occurring on the 15th day with a TIN removal rate of 80.26%). After 42 days with influent, ρ(NH4+-N) and ρ(NO2--N) respectively increased to 300 mg·L-1 and 396 mg·L-1, and the TIN removal rates of the three groups of reactors were respectively 78.96%, 84.92%, and 84.66%. Microbial community structure analysis showed that the relative abundances of ANAMMOX bacteria in the R2 and R3 reactor were respectively 6.85% and 6.06%, two to four times that in the R1 reactor. The predominant ANAMMOX bacteria in the sludge was Candidatus Jettenia, whose relative abundances in the three groups of reactors were respectively 1.62%, 5.74%, and 5.21%. The results show that ANAMMOX biofilm-granular sludge complex systems constructed by adding carriers can considerably shorten the time for recovering ANAMMOX sludge bacterial activity after long-term room temperature storage without substrate. The carriers effectively promoted the relative abundances of ANAMMOX bacteria in the reactors, whereas the promoting effect of comet fiber carrier was slightly more significant than that of the K3 carrier. |
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