| 厌氧氨氧化颗粒污泥的长期保藏及快速活性恢复 |
| 摘要点击 1998 全文点击 666 投稿时间：2020-11-08 修订日期：2020-11-30 |
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| 中文关键词 厌氧氨氧化(ANAMMOX) 长期保藏 活性恢复 有机碳源 反应途径 |
| 英文关键词 anaerobic ammonium oxidizing(ANAMMOX) long-term storage recovery organic carbon source reaction pathway |
| 作者 | 单位 | E-mail | | 李冬 | 北京工业大学城市建设学部, 水质科学与水环境恢复工程北京市重点实验室, 北京 100124 | lidong2006@bjut.edu.cn | | 刘名扬 | 北京工业大学城市建设学部, 水质科学与水环境恢复工程北京市重点实验室, 北京 100124 | | | 张杰 | 北京工业大学城市建设学部, 水质科学与水环境恢复工程北京市重点实验室, 北京 100124
哈尔滨工业大学环境学院, 城市水资源与水环境国家重点实验室, 哈尔滨 150090 | | | 曾辉平 | 北京工业大学城市建设学部, 水质科学与水环境恢复工程北京市重点实验室, 北京 100124 | |
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| 中文摘要 |
| 在4℃且无基质的条件下，将厌氧氨氧化颗粒污泥保藏230 d之后进行活性恢复，探究添加葡萄糖和丙酸钠两种有机碳源对于快速活性恢复的影响.经过230 d的长期保藏，厌氧氨氧化菌活性为0.013 g·（g·d）-1，仅为保藏前的6.02%，且平均粒径为135.05 μm，骤降至原来的38.23%，污泥解体、颜色发黑.在活性恢复阶段，R2和R3反应器分别添加葡萄糖和丙酸钠作为有机碳源，恢复结果显示，经过15 d的恢复，R1、R2和R3反应器的PN含量分别达到了126.30、188.86和168.82 mg·g-1，厌氧氨氧化菌活性均有所提升，分别达到了0.145、0.185和0.126 g·（g·d）-1，其中，添加葡萄糖作为有机碳源的R2反应器厌氧氨氧化菌活性最高，恢复到了保藏前的85.65%，且总氮去除率达到81.61%.第20d时，R1、R2和R3反应器中厌氧氨氧化颗粒污泥的粒径分别为289.81、359.66和314.37 μm，表明厌氧氨氧化颗粒污泥的长期保藏不是无法克服的难题，且在恢复阶段添加葡萄糖不仅可以有效提高EPS含量，促进颗粒生长和黏附，而且丰富了厌氧氨氧化的反应途径，使其在恢复阶段与其他细菌的底物竞争中占据优势，更快地恢复活性. |
| 英文摘要 |
| At 4℃ and with no substrate, the activity recovery of ANAMMOX granular sludge was examined after 230 days of storage, and the effect of adding two organic carbon sources (glucose and sodium propionate) on the recovery was explored. After 230 days of long-term storage, the activity of ANAMMOX bacteria was 0.013 g·(g·d)-1, which was just 6.02% of the baseline, and the average particle size was 135.05 μm, which was 38.23% lower. The sludge disintegration, black in color. In the activity recovery stage, the R2 and R3 reactors added glucose and sodium propionate as organic carbon sources. The recovery results showed that after 15 days of recovery, the PN content of the R1, R2, and R3 reactors reached 126.30, 188.86, and 168.82 mg·g-1, respectively, and the activity of the ANAMMOX bacteria was improved, reaching 0.145, 0.185, and 0.126 g·(g·d)-1, respectively. The R2 reactor with glucose as the organic carbon source had the highest ANAMMOX bacteria activity, which recovered 85.65% before preservation, and the total nitrogen removal rate reached 81.61%. On the 20th day, the particle sizes of the ANAMMOX granular sludge in the R1, R2, and R3 reactors were 289.81, 359.66, and 314.37 μm, respectively, indicating that the long-term preservation of ANAMMOX granular sludge is not an insurmountable problem. Furthermore, adding glucose during the recovery phase can not only effectively increase the EPS content and promote particle growth and adhesion, but also enrich the reaction pathways of ANAMMOX, enhancing recovery rates. |
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