| 铜尾矿白羊草重金属含量对叶际和根际真菌群落的影响 |
| 摘要点击 1925 全文点击 580 投稿时间：2020-04-27 修订日期：2020-05-15 |
| 查看HTML全文
查看全文 查看/发表评论 下载PDF阅读器 |
| 中文关键词 铜尾矿 叶际 根际 真菌群落 重金属 白羊草 |
| 英文关键词 copper tailing phyllosphere rhizosphere fungal community heavy metal Bothriochloa ischaemum |
|
| 中文摘要 |
| 植物的叶际和根际微生物与宿主植物之间存在复杂的相互关系，能促进植物的生长，增强宿主植物对逆境胁迫的耐受性.本实验选择铜矿区主要恢复植被白羊草为研究对象，采用高通量测序的方法，分析白羊草叶际和根际真菌群落结构与多样性差异，探究白羊草叶片和根部富集的重金属含量对叶际和根际真菌群落特征的影响.结果表明，在白羊草的根际和叶际区域，子囊菌门（Ascomycota）和担子菌门（Basidiomycota）均为优势菌门.白羊草根际真菌群落的多样性和丰富度显著高于叶际真菌.白羊草植株叶际和根际真菌多样性受到不同重金属的影响，白羊草叶际多样性主要受到叶片Zn和Cu含量的影响，而根Pb含量是影响根际真菌群落多样性的关键因子.此外，叶际子囊菌门的孢菌科与叶片Cd含量有极显著正相关关系，根际丛赤壳科与根Zn含量显著正相关，可作为重金属污染区域生态恢复的指示菌种.本研究为铜尾矿生态恢复过程中发掘和利用叶际或根际真菌资源提供科学依据，也为筛选重金属污染区生态修复的植物-微生物共生体提供基础. |
| 英文摘要 |
| There are complex interrelationships between plant microorganisms (phyllosphere and rhizosphere) and host plants, which can promote plant growth and enhance the tolerance of host plants to stress. In this study, we selected the dominant species Bothriochloa ischaemum as the research subject in a copper tailings dam. Using high-throughput sequencing, we investigated the structures of the fungal communities and diversities in the phyllosphere and rhizosphere of B. ischaemum. This study also explored the effects of heavy metal content on fungal community characteristics. The results showed that Ascomycota and Basidiomycota were the dominant phyla in the phyllosphere and rhizosphere of B. ischaemum. The diversities and richness of the rhizosphere fungal community were higher than that of the phyllosphere fungal community. The diversities of rhizosphere and phyllosphere fungal communities was affected by different heavy metals. Phyllosphere fungal diversity was mainly affected by the content of Zn and Cu in leaves, and the content of Pb in roots was the key factor affecting the diversity of the rhizophere fungal community. Furthermore, Pleosporaceae had a very significant positive correlation with Cd in the phyllosphere, and Nectriaceae had a significant positive correlation with Zn in the rhizosphere. These fungal communities could be used as indicators of ecological recovery in areas with heavy metal pollution. The results could provide an ecological basis for the exploration and utilization of phyllosphere or rhizosphere fungi resources during ecological restoration processes. This study also provides guidance for selecting the plant-microbial symbionts during ecological restoration in areas with heavy metal pollution. |
