| 游离羟胺对两种典型亚硝态氮氧化菌活性的影响 |
| 摘要点击 1174 全文点击 382 投稿时间：2019-11-13 修订日期：2020-01-02 |
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| 中文关键词 硝化杆菌 硝化螺菌 相对活性 羟胺 游离羟胺 |
| 英文关键词 Nitrobacter Nitrospira relative activity hydroxylamine free hydroxylamine |
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| 中文摘要 |
| 为考察羟胺对两种典型亚硝态氮氧化菌（NOB）活性的影响，分别以富含硝化杆菌（Nitrobacter）和富含硝化螺菌（Nitrospira）的污泥作为研究对象，基于批次试验，分别考察了相同pH、不同羟胺浓度梯度和相同羟胺浓度、不同pH梯度条件下，羟胺对两种NOB的抑制效果.结果表明在相同pH条件下，Nitrobacter的活性随羟胺浓度的升高而降低；在相同羟胺浓度（HA=5mg·L-1）条件下，羟胺在较高pH环境（pH≥7.5）中产生更多的游离羟胺（FHA），会对Nitrobacter产生更好的抑制效果，低pH环境（pH≤7）中离子态羟胺的存在可能会促进Nitrobacter的活性.羟胺对Nitrospira的抑制效果有限，当pH=7.5，羟胺浓度=45mg·L-1时，Nitrospira的相对活性为82%.分别采用NOB生长速率动力学模型和非底物抑制线性方程描述FHA对Nitrobacter和Nitrospira活性的影响，其可决系数R2分别为0.90和0.94，FHA可能是抑制Nitrobacter和Nitrospira活性的主要原因. |
| 英文摘要 |
| The sludge from enrichment of Nitrobacter and Nitrospira was used as a research object and batch tests were performed. The inhibitory effects of hydroxylamine on Nitrobacter and Nitrospira under the same pH and different hydroxylamine concentration gradients, the same hydroxylamine concentration, and different pH gradients were investigated. The results showed that under the same pH condition, the activity of Nitrobacter decreased with increasing hydroxylamine concentration. Under the same hydroxylamine concentration (HA=5 mg·L-1) at a higher pH environment (pH ≥ 7.5), hydroxylamine produced more free hydroxylamine (FHA) and the inhibitory effect on Nitrobacter was improved. At a low pH environment (pH≤7), ionic hydroxylamine promoted the activity of Nitrobacter. The inhibitory effect of hydroxylamine on Nitrospira was limited. When pH=7.5 and hydroxylamine concentration was 45 mg·L-1, the relative activity of Nitrospira was 82%. The NOB growth rate kinetics model and the non-substrate inhibition linear equation were used to describe the effect of FHA on Nitrobacter and Nitrospira activity. The coefficient of determination R2 was 0.90 and 0.94, respectively. FHA may be the main reason for inhibiting the activity of Nitrobacter and Nitrospira. |
