| 淀粉废水处理系统中活性污泥的微生物群落结构及多样性分析 |
| 摘要点击 2330 全文点击 713 投稿时间：2019-05-16 修订日期：2019-07-30 |
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| 中文关键词 细菌群落结构 生物多样性 活性污泥 高通量测序 淀粉废水 |
| 英文关键词 bacterial community structure biodiversity activated sludge high-throughput sequencing waste water containing starch |
| 作者 | 单位 | E-mail | | 赵婷婷 | 北京师范大学环境学院, 北京 100875 | 954962316@qq.com | | 乔凯 | 北京师范大学环境学院, 北京 100875 | | | 王蕾 | 北京师范大学环境学院, 北京 100875 | | | 张伟 | 北京师范大学环境学院, 北京 100875 | | | 竺建荣 | 北京师范大学环境学院, 北京 100875 | zjrtshua@hotmail.com | | 唐一 | 南方科技大学工程技术创新中心, 北京 100083
北京南科大蓝色科技有限公司, 北京 100083 | | | 胡滨 | 南方科技大学工程技术创新中心, 北京 100083 | | | 王香莲 | 南方科技大学工程技术创新中心, 北京 100083
北京南科大蓝色科技有限公司, 北京 100083 | | | 杨静 | 山东西王糖业有限公司, 邹平 256209 | | | 景在涛 | 山东西王糖业有限公司, 邹平 256209 | |
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| 中文摘要 |
| 为探究淀粉废水处理活性污泥中微生物的群落结构及多样性，基于Illumina MiSeq高通量测序方法，分析了不同运行阶段的A/O处理系统中活性污泥的微生物群落与多样性组成.结果表明，A/O系统中处理淀粉生产废水的活性污泥在同一种废水下微生物群落结构总体比较稳定，优势细菌主要为变形菌门（Proteobacteria）、拟杆菌门（Bacteroidetes）、绿弯菌门（Chloroflexi）、厚壁菌门（Firmicutes）和放线菌门（Actinobacteria）等；最重要优势细菌类群为变形菌门（45.66%~66.30%），其中γ-亚纲细菌是其主要成员，占比36.38%~66.65%.优势拟杆菌门主要成员鞘脂杆菌纲在污泥沉降性能较好时其占比下降，但绿弯菌门主要成员厌氧绳菌纲在污泥沉降性能较好时其占比明显增加，变化趋势正好相反，或许它们二者之间的耦合变化与污泥沉降性能变化密切相关.活性污泥样品中存在大量特殊功能菌群，它们在活性污泥的污染物分解和氮磷去除中发挥重要作用. |
| 英文摘要 |
| This study analyzes the microbial community and diversity composition of activated sludge in anoxic/oxic (A/O) treatment systems at different operation stages using Illumina MiSeq high-throughput sequencing to investigate the microbial community structure and diversity in activated sludge for starch wastewater treatment. The experimental results showed that the microbial community structure of activated sludge for starch production wastewater treatment in A/O systems was quite stable under the same wastewater condition, and that the dominant bacteria of the activated sludge were Proteobacteria, Bacteroidetes, Chloroflexi, Firmicutes, and Actinobacteria. The most important dominant bacterial group was Proteobacteria (45.66%-66.30%), of which γ-subclass bacteria were the main member and occupied 36.38%-66.65%. The proportion of Sphingobacteria, the main member of the Bacteroidetes, decreased when the sludge settling performance was better, but the proportion of Anaerolineae, the main member of Chloroflexi, increased significantly when the sludge sedimentation performance was better. These changes may have been closely related to the behavior of sludge settleability. There were a large number of functional bacteria in the activated sludge, which played an important role in the degradation of pollutants and in nitrogen and/or phosphorus removal. |
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