| PFOS前体物质(PreFOSs)降解菌的分离鉴定及其降解特性 |
| 摘要点击 1561 全文点击 615 投稿时间：2017-11-30 修订日期：2018-01-02 |
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| 中文关键词 PFOS前体物质(PreFOSs) 微生物降解 生丝微菌属 分离鉴定 降解特性 |
| 英文关键词 perfluorooctane sulfonate (PFOS) precursors (PreFOSs) biodegradation Hyphomicrobium sp. isolation and identification degradation characteristics |
| 作者 | 单位 | E-mail | | 赵淑艳 | 大连理工大学食品与环境学院, 工业生态与环境工程教育部重点实验室, 盘锦 124221 | Zhaoshuyan@dlut.edu.cn | | 周涛 | 大连理工大学食品与环境学院, 工业生态与环境工程教育部重点实验室, 盘锦 124221 | | | 王博慧 | 大连理工大学食品与环境学院, 工业生态与环境工程教育部重点实验室, 盘锦 124221 | | | 梁田坤 | 大连理工大学食品与环境学院, 工业生态与环境工程教育部重点实验室, 盘锦 124221 | | | 柳丽芬 | 大连理工大学食品与环境学院, 工业生态与环境工程教育部重点实验室, 盘锦 124221 | |
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| 中文摘要 |
| 从氟化工厂附近土壤中分离出1株能以全氟辛烷磺酸前体物质（PreFOSs）为唯一碳源和能源生长的降解菌PF1，经形态观察及16S rDNA基因序列分析，初步鉴定该菌为生丝微菌属（Hyphomicrobium sp.）.在温度为30℃、pH为7.0~7.2条件下，菌株PF1对全氟辛基磺酰胺（PFOSA）和N-乙基全氟辛基磺酰胺（N-EtFOSA）48 h降解率分别为14.6%和8.2%，对PFOS无降解能力.对降解产物进行检测和分析，结果表明PFOSA的降解产物为PFOS；N-EtFOSA能被降解生成PFOSA和PFOS，同时也产生少量的全氟辛基磺酰胺乙酸（FOSAA）.由此推断PreFOSs降解途径，在菌株PF1的作用下，PFOSA脱去氨基直接转化成PFOS.N-EtFOSA主要有2种降解途径：①N-EtFOSA脱乙基产生PFOSA，PFOSA再进一步脱氨基生成PFOS，此为主要途径；② N-EtFOSA中的N-乙基被氧化成乙酸基生成FOSAA，FOSAA进一步脱去乙酸基生成PFOSA，并最终脱氨基生成PFOS. |
| 英文摘要 |
| Transformation of perfluorooctane sulfonate (PFOS) precursors (PreFOSs) is considered an additional source of PFOS in the environment and biota. A PreFOSs-degrading bacterium PF1, which was able to utilize PreFOSs as the sole carbon and energy source for growth, was isolated from contaminated soil collected from the surroundings of a fluoride factory. According to its morphology and 16S rDNA gene sequence analysis, strain PF1 was identified as Hyphomicrobium sp. The degradation rates of perfluorooctane sulfonamide (PFOSA) and N-ethyl perfluorooctane sulfonamide (N-EtFOSA) by PF1 were 14.6% and 8.2% (30℃; pH=7.0-7.2), respectively, whereas PF1 was unable to degrade PFOS. PFOSA could be biodegraded to PFOS. N-EtFOSA could be biodegraded to perfluorooctane sulfonamide acetic acid (FOSAA), PFOSA, and PFOS; PFOS was the predominant metabolite. Based on the above analysis, the proposed metabolic pathway of PFOSA by strain PF1 is deamination to form PFOS. Two possible degradation pathways are proposed for N-EtFOSA: ① deethylation of N-EtFOSA to produce PFOSA, followed by deamination to form PFOS, and ②oxidation of N-EtFOSA to FOSAA followed by sequential dealkylation to produce PFOSA, and then transformation to PFOS by deamination. |
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