| 耐冷嗜碱蒙氏假单胞菌H97的鉴定及其好氧反硝化特性 |
| 摘要点击 1635 全文点击 502 投稿时间：2017-09-28 修订日期：2018-01-12 |
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| 中文关键词 耐冷 嗜碱 蒙氏假单胞菌 脱氮 反硝化特性 |
| 英文关键词 hypothermia alkaliphilic Pseudomonas monteilii nitrogen removal denitrification characteristics |
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| 中文摘要 |
| 结合形态、磷脂脂肪酸及16S rRNA基因序列分析鉴定了分离自贵州冬水田的好氧反硝化菌株H97，采用模拟废水探讨了不同温度、接菌量、C/N、初始pH和碳源种类对菌株H97反硝化能力的影响.结果表明，菌株H97为蒙氏假单胞菌（Pseudomonas monteilii），目前国内外尚无蒙氏假单胞菌具有耐冷嗜碱好氧反硝化作用的研究报道，是对好氧反硝化菌的补充；该菌的最适脱氮条件为15℃、pH 9.0、C/N 15、接种量1.5×106 CFU·（100 mL）-1，碳源丁二酸钠.此外，菌株H97在pH为7.0~11.0时，均表现出良好的脱氮能力，对硝酸盐氮和总氮的去除率分别可达91.21%和79.10%以上，初始pH为12.0时，对硝酸盐氮和总氮的去除率仍分别达64.75%和36.78%，表现出了较强耐碱能力.在初始硝酸盐氮浓度为50.0 mg·L-1和最适脱氮条件下，菌株H97在48 h内对硝酸盐氮和总氮的去除率可达97.69%和96.32%.同时，H97对温度适应范围广，在15~40℃均具有较强好氧反硝化能力，是1株耐冷嗜碱好氧反硝化细菌，在碱性氮污染水体处理中具有较好的应用潜力. |
| 英文摘要 |
| Low temperatures and high pH generally inhibit bio-denitrification. Thus, it is important to explore psychrotrophic and alkali-resistant microorganisms for nitrogen degradation. This study mainly focused on the identification of an alkaliphilic strain and preliminary exploration of its denitrification characteristics. Based on morphological observations, phospholipid fatty acids and 16S rRNA gene sequence analysis, strain H97, which was isolated from the winter paddy field in Guizhou province, was identified as Pseudomonas monteilii. Till date, there were few reports about the denitrification characteristics of Pseudomonas monteilii. The effects of environmental factors such as temperature, inoculation quantity, C/N ratio, initial pH, and carbon source were investigated using simulated wastewater. The optimum conditions for nitrate and total nitrogen removal by H97 were: inoculum size 1.5×106 CFU·(100 mL)-1; initial pH 9.0; C/N=15; 15℃; and sodium succinate as the carbon source. The nitrate and total nitrogen removal efficiencies were 97.69% and 96.32%, respectively, at optimum conditions with an initial nitrate nitrogen concentration of 50.0 mg·L-1. The temperature experiments indicated that the optimal temperature for highest nitrogen removal efficiency was 15℃, and that the strain H97 could survive in a wide range of 15-40℃. Additionally, the nitrate and total nitrogen efficiencies at the initial pH value of 7.0-11.0 were 91.21% and 79.10%, respectively, and the denitrification capacity then decreased to 64.75% at the initial pH 12.0. These results indicated that strain H97 showed cold and alkali resistance, which suggests an application potential for the treatment of alkaline nitrogen polluted water in the southern winter. |
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