| 深水型水库热分层诱导水质及真菌种群结构垂向演替 |
| 摘要点击 2616 全文点击 921 投稿时间：2017-06-30 修订日期：2017-08-26 |
| 查看HTML全文
查看全文 查看/发表评论 下载PDF阅读器 |
| 中文关键词 深水型水库 热分层 真菌种群 高通量测序 |
| 英文关键词 deep reservoir thermal stratification fungal community high-throughput sequencing |
|
| 中文摘要 |
| 水体真菌种群在调控水库生态系统健康过程中发挥重要作用，为探明热分层诱导深水型水库水体真菌种群结构垂向演替特征，采集金盆水库主库区0.5、10、25、40、60和70 m水深水样，分析水质参数、并运用高通量DNA测序技术诊断水库水体真菌种群结构垂向异质性.结果表明，金盆水库表层水温最高为22.33℃，底层最低为7.21℃（P<0.05）.水体溶解氧（DO）随水深增加显著降低（P<0.05），电导率、总磷（TP）和铁含量随着水深增加显著升高（P<0.05），垂向形成稳定的变温层、斜温层和等温层结构.水体真菌种群高通量DNA测序共发现1247个OTUs，隶属4门14纲39属，主要包括接合菌门（Zygomycota）、担子菌门（Basidiomycota）、子囊菌门（Ascomycota）和壶菌门（Chytridiomycota）.等温层水体真菌种群Shannon指数和Chao 1多样性指数最高，分别为3.45和360，显著高于斜温层水体（P<0.05）.红酵母属（Rhodotorula，27.23%）、链格孢属（Alternaria，24.28%）、分子孢子菌属（Cladosporium，22.98%）、链格孢属（Alternaria，32.00%）、亚隔孢壳属（Didymella，17.47%）和分子孢子菌属（Cladosporium，28.17%）分别是0.5、10、25、40、60、70 m的优势菌属，不同水深均存在大量未知真菌（Unclassified）.热图（heat map）结果表明，热分层期间金盆水库水体真菌种群结构垂向分布差异显著、不同水层具有不同的真菌种群结构.主成分分析（PCA）表明水温、DO、TP、电导率是调控水体真菌种群结构垂向异质性的主要水质因子.该研究结果为水源水库热分层期间水质与真菌群落的偶联机制提供科学依据. |
| 英文摘要 |
| Freshwater fungal community composition plays pivotal roles in sustaining the function and health of drinking water reservoir ecosystems. To investigate the vertical evaluative characteristics of an aquatic fungal community under conditions of stable thermal stratification, water samples were collected at 0.5, 10, 25, 40, 60 and 70 m depths of Jinpen Reservoir during thermal stratification. Fungal community composition was determined using a high-throughput DNA sequencing technique and combined with water quality parameters. The results showed that the epilimnion, metalimnion, and hypolimnion water layers were formed in Jinpen Reservoir. The temperature decreased steadily from the surface at 22.33℃ to the bottom with 7.21℃ (P<0.05). The dissolved oxygen (DO) concentration decreased significantly from the surface to the bottom (P<0.05). The conductivity and the concentration total phosphorus and iron increased significantly from the surface to the bottom (P<0.05). The high-throughput DNA sequencing revealed a total of 1247 operational taxonomic units (OTUs), which were affiliated with four phylum, 14 classes, and 39 genera, including Zygomycota, Basidiomycota, Ascomycota, and Chytridiomycota. The highest Shannon diversity and Chao 1 were 3.45 and 360 at 60 m, respectively. The Shannon diversity and Chao 1 were significantly higher in the hypolimnion than in the metalimnion (P<0.05). Rhodotorula (27.23%), Alternaria (24.28%),Cladosporium (22.98%), Alternaria (32.00%), Didymella (17.47%), and Cladosporium (28.17%) were the dominant species at 0.5 m, 10 m, 25 m, 40 m, 60 m, and 70 m, respectively. There are a number of unclassified fungi at different water depths. The heat map profile indicated significant differences in the vertical distribution of the fungal community composition in Jinpen Reservoir. A principle component analysis (PCA) indicated that water temperature, DO, TP, and conductivity had dramatic influences on the vertical distribution of the fungal community composition. The results provide new insights on the relationship between water quality and fungal community composition during reservoir thermal stratified periods. |
|
|
|
