| 关帝山森林土壤真菌群落结构与遗传多样性特征 |
| 摘要点击 4178 全文点击 1592 投稿时间：2016-11-12 修订日期：2016-12-30 |
| 查看HTML全文
查看全文 查看/发表评论 下载PDF阅读器 |
| 中文关键词 关帝山 高通量测序 真菌群落结构 针叶林土壤 |
| 英文关键词 Guandi Mountains high-throughput sequencing fungi community structure coniferous forest soil |
|
| 中文摘要 |
| 土壤微生物群落驱动土壤碳、氮、磷、硫生物地球化学循环，在维持土壤碳汇和生态系统功能等方面发挥重要的作用.分析环境与空间因素在寒温性针叶林土壤微生物群落构建中的作用，可以为区域森林生态系统管理措施的制定提供理论依据.本文利用Illumina高通量测序技术分析了关帝山庞泉沟自然保护区华北落叶松（Larix principis-rupprechtii）林、青杄（Picea wilsonii）林、油松（Pinus tabulaeformis）林以及桦树（Betula spp.）林这4种林型的5个土壤真菌群落（LpMC1、LpMC2、PwMC、PtMC和BMC）的结构.同时测定林下土壤环境因子及林下植被多样性，分析真菌群落的结构与植被和土壤环境因子的相关性.结果表明：①5个样地中共有7个真菌门和33个优势真菌属；②冗余分析结果表明：土壤pH、温度、含水率、全氮含量、NH4+含量、全碳含量、蔗糖酶活性、脲酶活性、林下植被优势度和均匀度与土壤真菌群落结构显著相关；③聚类分析和PCA分析结果表明，森林植被类型、土壤环境因子和林下植被对土壤真菌群落结构影响显著.④空间变量（PCNM）分析结果表明，在局域尺度（local scale）上扩散限制对研究区域真菌群落构建的影响不显著.本研究区森林土壤真菌群落结构主要受到环境选择（土壤pH、温度、含水率、全氮含量、NH4+含量、全碳含量、蔗糖酶活性、脲酶活性、林下植被优势度和均匀度、森林类型）的显著影响. |
| 英文摘要 |
| Soil microorganisms drive the biogeochemical process of carbon, nitrogen, phosphorus and sulfur, and play a key role in maintaining soil carbon sink and ecosystem function. The study on effects of environmental and spatial factors on the structure of microbial community in boreal coniferous forest soil will provide theoretical basis for making management measures in local forest ecosystem. Our research analyzed five soil fungi communities (LpMC1, LpMC2, PwMC, PtMC, and BMC) in four forest types, including Larix principis-rupprechtii forest, Picea wilsonii forest, Pinus tabulaeformis forest and Betula spp. forest, respectively, in Pangquangou Nature Reserve in Guandi Mountains with Illumina high-throughput sequencing technology. Meanwhile, soil environmental factors and diversity of undergrowth plants were determined to analyze the relationship between fungi community structure and vegetation as well as soil environmental factors. The results showed that:①There were seven eumycota and thirty-three advantageous fungal genera in the five sample sites; ②Redundancy analysis results showed that soil pH, temperature, moisture, total nitrogen, the content of NH4+, total carbon, invertase activity, urease activity, undergrowth dominance and evenness were significantly associated with soil fungi community structure; ③Cluster analysis and principal component analysis showed that forest vegetation type, soil environmental factors and undergrowth had significant effects on soil fungi community structure; ④The results of PCNM analysis showed that at a local scale, dispersal limitation had no significant influence on fungi community structure in the study area. The forest soil fungi community structure in the study area was significantly affected by environmental selection (soil pH, temperature, moisture, total nitrogen, the content of NH4+, total carbon, invertase activity, urease activity, undergrowth dominance and evenness, forest type). |
|
|
|
