| 一种新型生物膜法除磷工艺中聚磷菌的富集培养过程 |
| 摘要点击 1925 全文点击 700 投稿时间：2016-07-13 修订日期：2016-08-17 |
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| 中文关键词 生物膜 除磷 聚磷菌 尼龙填料 富集 FISH |
| 英文关键词 Biofilm phosphorus removal PAOs nylon filler enrichment FISH |
| 作者 | 单位 | E-mail | | 郑莹 | 苏州科技大学环境科学与工程学院, 苏州 215009
苏州科技大学环境生物技术研究所, 苏州 215009 | 350449185@qq.com | | 潘杨 | 苏州科技大学环境科学与工程学院, 苏州 215009
苏州科技大学环境生物技术研究所, 苏州 215009
江苏省环境科学与工程重点实验室, 苏州 215009 | panyang@mail.usts.edu.cn | | 周晓华 | 苏州科技大学环境科学与工程学院, 苏州 215009 | | | 廖烜弘 | 苏州科技大学环境科学与工程学院, 苏州 215009
苏州科技大学环境生物技术研究所, 苏州 215009 | | | 孟璇 | 苏州科技大学环境科学与工程学院, 苏州 215009
苏州科技大学环境生物技术研究所, 苏州 215009 | | | 夏健伟 | 苏州市环境应急与事故调查中心, 苏州 215002 | |
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| 中文摘要 |
| 采用挂式尼龙作为生物载体的新型生物膜反应器处理合成废水，探讨短时间内在该常规生物膜上富集培养高浓度聚磷菌的可行性，并从反应器运行效率、除磷速率以及聚磷菌的富集状态等方面进行验证.反应器启动运行10 d后，好氧阶段正磷酸盐去除率稳定在95%以上，COD出水浓度均在50 mg·L-1以下，并在该处理水平稳定运行了50 d.运行培养48 d后，吸磷及释磷速率由相同的3.4 mg·（L·h）-1分别提高到8 mg·（L·h）-1和6 mg·（L·h）-1，好氧和厌氧周期由相同的6 h分别缩短到2 h和3 h.运行培养50 d经荧光原位杂交法（FISH）测定，污泥中聚磷菌的丰度从原泥的48.96%提高到70%，杂交图中的聚磷菌以大块团聚态出现，由直接显微镜法测得生物膜厚度约为28.9 μm，证明生物膜上聚磷菌群已处于动力学增长末期即生物膜已经成熟.经过50 d的强化培养，能够在常规尼龙填料上富集占总菌70%的高浓度聚磷菌，使得本反应器能高效去除污水中的磷与有机物. |
| 英文摘要 |
| Using the hanging nylon as a biological carrier,a novel biofilm reactor was adopted to treat synthetic wastewater,and the feasibility of cultivating and enriching a high concentration of PAOs on this conventional biofilm within a short time was investigated,which was proved from the aspects of reactor's operational efficiency,the rate of phosphorus removal and the condition of PAOs enrichment.After 10d of operation,the rate of orthophosphate removal was higher than 95% in aerobic phase and the concentration of effluent COD was 50 mg·L-1 or less in the reactor,which was operated steadily for 50 d at this treatment level;after 48 d of operation,the reactor's phosphorus uptake rate and release rate were increased from 3.4 mg·(L·h)-1 and 3.4 mg·(L·h)-1to 8 mg·(L·h)-1 and 6 mg·(L·h)-1,respectively,and the aerobic and anaerobic cycles were shortened from equally 6 h to 2 h and 3 h,respectively.The fluorescence in situ hybridization (FISH) test found that the PAOs' abundance was increased from the original 48.96% to 70% on the 50th day,meanwhile the PAOs showed reunite chunk state in hybrid figure,the thickness of biofilm measured by direct microscopic process was about 28.9 μm,which all proved that the PAOs in biofilm were at the end of the growth kinetics and the biofilm was mature.By hardening culture for 50d,a high concentration of 70% in full organisms of PAOs could be enriched in the conventional nylon filler,enabling the reactor to show a high efficiency in removal of phosphorus and organic matter from sewage. |
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