| 几种高硅质矿物细颗粒的A549细胞毒性对比 |
| 摘要点击 1387 全文点击 724 投稿时间：2016-03-22 修订日期：2016-06-08 |
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| 中文关键词 石英 绢云母 钠长石 蒙脱石 A549细胞 毒性效应 |
| 英文关键词 quartz sericite albite montmorillonite A549 cells toxic effect |
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| 中文摘要 |
| 以大气颗粒物成分中主要矿物相：石英、绢云母、钠长石和蒙脱石这4种高硅质矿物细颗粒为研究对象，检测其对A549细胞存活率、膜损伤及炎性因子释放的影响，旨在对比分析几种高硅质矿物细颗粒的毒性大小，并从颗粒物物理化学性质角度阐述其细胞毒性作用机制.结果表明，几种硅质细颗粒对A549细胞存活率的影响：蒙脱石 > 绢云母≥石英 > 钠长石.细胞存活率与矿物颗粒样品的SiO2含量之间相关性较差，与Fe2O3含量存在较好相关性，Fe2O3含量越高毒性越大.高硅质矿物颗粒共培养环境中H2O2释放量与样品中Fe2O3含量呈正相关关系.蒙脱石组细胞受H2O2影响最大，石英和钠长石组细胞受粉体释放H2O2影响较小.所检测的几种高硅质矿物颗粒不同程度地造成细胞膜损伤，且均可触发A549细胞释放TNF-α或IL-6，进一步激发免疫反应的产生，但不同类型的高硅质矿物颗粒促发炎性反应不同.高硅质矿物细颗粒的成分结构并不是影响其生物活性的唯一因素，粉体的外在形态、表面活性基团、溶解性、吸附和离子交换特性等对细胞的存活率、细胞膜损伤、液相自由基的释放及炎性反应的影响同样不容忽视. |
| 英文摘要 |
| Considering the high contents of minerals and the potential health risk of mineral dusts to human and the environment, this paper was aimed to figure out the toxic effect and mechanism of four common mineral particles (quartz, albite, sericite, and montmorillonite). Cytotoxicity assays for cell viability (MTT assay), membrane integrity (LDH assay), oxidative stress (H2O2 assay) and inflammatory cytokines (TNF-α and IL-6 assay) were applied. The results showed the influence of these mineral particles on A549 cell viability followed the order of momtmorillonite > cericite≥quartz > albite. There was no obvious relation between cell viability and the content of SiO2, however, good linear correlation with the content of iron, and the cytotoxicity of mineral dusts was strengthened with increasing iron content. Mineral dusts generated H2O2 in cell or cell-free systems. In particular, H2O2 exhibited a linear correlation with the iron content, which meant that iron in the mineral dusts played an important role in the generation of reactive radical. Among those samples, oxidative stress induced by montmorillonite was distinctly stronger, while there was negligible influence induced by quartz and albite. Besides, all the tested samples induced damage to A549 cell membrane, and triggered the release of TNF-α or IL-6, but differed by the kinds of mineral dusts. In conclusion, composition and structure directly affected, but were not the only factors that contributed to the biological activity of mineral dusts, the evaluation of cell viability, membrane damage, free radicals and inflammatory reaction induced by mineral dusts should take the external morphology, surface active groups, solubility, adsorption and ion exchange properties into consideration. |
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