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低浓度氨氮废水单级自养脱氮EGSB反应器的快速启动
摘要点击 2379  全文点击 1106  投稿时间:2016-01-08  修订日期:2016-03-21
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中文关键词  单级自养脱氮工艺  低浓度氨氮废水  启动  颗粒污泥  膨胀颗粒污泥床反应器
英文关键词  one-stage autotrophic nitrogen removal process  low concentration of ammonia wastewater  start-up  granular sludge  expanded granular sludge bed reactor
作者单位E-mail
顾书军 重庆大学城市建设与环境工程学院, 重庆 400045 shujungu@126.com 
方芳 重庆大学城市建设与环境工程学院, 重庆 400045 fangfangcq@cqu.edu.cn 
李凯 重庆大学城市建设与环境工程学院, 重庆 400045  
刘勇 重庆大学城市建设与环境工程学院, 重庆 400045  
郭劲松 中国科学院重庆绿色智能技术研究院, 重庆 401122  
陈猷鹏 中国科学院重庆绿色智能技术研究院, 重庆 401122  
蒋甫阳 重庆大学城市建设与环境工程学院, 重庆 400045  
中文摘要
      将单级自养脱氮生物膜污泥作为种泥接种于膨胀颗粒污泥床反应器中,并对该反应器进行启动. 在温度为(30±2)℃,pH值为7.8~8.2,DO为0.2~1.1 mg·L-1,上升流速为2.0~4.0m·h-1的条件下,进行了低浓度氨氮(60~100 mg·L-1)废水的单级自养脱氮工艺快速启动研究. 结果表明,经过83 d的运行,反应器历经适应期、提高期和稳定期后,稳定运行阶段NH4+-N、TN去除率分别达到99.4%和80.7%. 通过控制回流比和提高进水氨氮负荷维持了稳定的低DO状态,有效抑制了硝化细菌的生长,而部分亚硝化反应和厌氧氨氧化反应为主导反应并能保持高效、稳定的脱氮效果,成功实现了在膨胀颗粒污泥床反应器中单级自养脱氮过程的启动. 颗粒污泥平均粒径从174 μm增大到296 μm. 扫描电镜显示颗粒污泥表面光滑,微生物以球菌、短杆菌为主. FISH结果显示亚硝化细菌分布在颗粒污泥表面,厌氧氨氧化菌分布在颗粒污泥内部. 反应器中构建了稳定的自养脱氮颗粒污泥系统.
英文摘要
      Biofilm coming from a reactor in which One-stage Autotrophic Nitrogen Removal Process exists was selected as inoculum in an expanded granular sludge bed reactor. A potential fast start-up procedure was tested in this research. Wastewater with low ammonium concentration between 60-100 mg·L-1 was applied. The results showed that a One-stage Autotrophic Nitrogen Removal Process was successfully established in 83 days under the following conditions: temperature at (30±2)℃ , pH at 7.8-8.2, dissolved oxygen (DO) at 0.2-1.1mg·L-1 and upflow velocity at 2.0-4.0m·h-1. After the 83-day operation, the removal efficiencies of NH4+-N and TN were 99.4% and 80.7%, respectively. By controlling the reflux ratio and increasing the NH4+-N load, the reactor could maintain a stable state of low DO concentration. Nitrification and anaerobic ammonium oxidation became main reactions that maintained efficient and stable nitrogen removal performance. In addition, nitrite-oxidizing bacteria (NOB) were inhibited. In the start-up process, the average particle diameter of granular sludge increased from 174 to 296 μm. Moreover, scanning electron microscopy showed that the surface of granular sludge was smooth, and most microorganisms were bevibacteria and cocci. Finally, a fluorescence in situ hybridization experiment showed that ammonium oxidation bacteria and anaerobic ammonium oxidation bacteria were distributed on the surface and in the inner space of granular sludge, respectively. The research indicated that a stable autotrophic nitrogen removal granular sludge system was quickly established in the sludge bed.

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