| 鸢尾对石油烃污染土壤的修复以及根系代谢分析 |
| 摘要点击 1886 全文点击 1060 投稿时间：2015-09-14 修订日期：2015-12-03 |
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| 中文关键词 植物修复 石油烃 污染土壤 鸢尾 根系 代谢分析 |
| 英文关键词 phytoremediation total petroleum hydrocarbons (TPHs) contaminated soil Iris pseudacorus L. roots metabolic analysis |
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| 中文摘要 |
| 本研究采用温室盆栽方式,探讨了野生花卉鸢尾(Iris pseudacorus L.)对大港油田石油烃污染土壤的修复能力,并对石油烃暴露下鸢尾根系代谢组学进行了分析. 结果表明,鸢尾对石油烃含量≤40000 mg ·kg-1具有良好的耐性; 鸢尾对高含量石油烃污染土壤的修复效果比较显著,当土壤石油烃含量为10000、20000和40000 mg ·kg-1时,其去除率分别为42.1%、33.1%和31.2%,明显高于空白对照组(31.8%、21.3%和11.9%)(P<0.05). 通过根系扫描,发现在10000、20000和40000 mg ·kg-1的石油烃暴露下,根系比表面积均高于空白对照组. 经聚类分析发现根系代谢从整体上发生了不同程度的变化,同时某些代谢物的含量或种类也发生了极显著的变化(P<0.001). 通过偏最小二乘判别分析法(PLS-DA)发现11种代谢物(VIP值>1.2),它们对根系比表面积的改变起到实质性的贡献. 其中,5种与根系比表面积呈正相关,分别为草酸、乳酸、延胡索酸、磷酸和丙二酸,而与葡萄糖酸、尿苷、丁酸、麦芽糖、亚油酸和苯丙氨酸呈负相关. 可见,鸢尾用于修复高含量石油烃污染土壤具有实践上的可行性,并且关于根系代谢分析有利于更好地理解植物对石油烃污染土壤的代谢响应并揭示其修复机制. |
| 英文摘要 |
| In this study, we performed a greenhouse pot-culture experiment to investigate the potential of a wild ornamental plant Iris pseudacorus L. in remediating petroleum contaminated soils from the Dagang Oilfield in Tianjin, China. The results suggested that Iris pseudacorus L. had great resistance to ≤40000 mg ·kg-1 of total petroleum hydrocarbons (TPHs). The removal rate of TPHs with concentrations of 10000 mg ·kg-1, 20000 mg ·kg-1 and 40000 mg ·kg-1 in soils by Iris pseudacorus L. was 42.1%, 33.1%, 31.2%, respectively,much higher than those in the corresponding controls (31.8%, 21.3%, 11.9%, respectively) (P<0.05). The root specific surface area of Iris pseudacorus L. was determined by the root scanner. The results suggested that TPHs with concentrations of 10000 mg ·kg-1, 20000 mg ·kg-1 and 40000 mg ·kg-1 in soils increased the root specific surface area comparing with the controls. Additionally, the metabolic analysis showed that root metabolism changed to different degrees under the stress of TPHs, and the levels or species of metabolites had a significant change (P<0.001). Furthermore, the results showed that 5 of 11 metabolites(VIP value>1.2)with the root specific surface area from the PLS-DA model analysis, including ethanedioic acid, lactic acid, 2-butenedioic acid, phosphate and propanedioic acid, were positively correlated with the root specific surface area, but the others, gluconic acid, uridine, butanoic acid, maltose, 9,12-octadecadienoic acid, phenylalanine, were negatively correlated with it. In conclusion, using Iris pseudacorus L. to remediate petroleum contaminated soils is feasible, and the metabolic analysis in roots is useful to better understand the metabolic response of plants exposure to petroleum contaminated soils, and then reveals its remediated mechanisms. |
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