| 乙腈和正己烷对环境特征污染物免疫传感分析的影响 |
| 摘要点击 1713 全文点击 922 投稿时间：2015-06-20 修订日期：2015-09-01 |
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| 中文关键词 光纤免疫传感器 倏逝波 双酚A 乙腈 正己烷 免疫分析 |
| 英文关键词 optical-fiber immunosensor evanescent bisphenol A acetonitrile n-hexane immunoassay |
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| 中文摘要 |
| 利用前期研发的倏逝波全光纤免疫传感系统,使用间接竞争免疫模式对双酚 A(bisphenol A,BPA)进行了定量分析,其检测灵敏度可达 0.2 μg ·L-1,线性检测区间为 0.3~33.4 μg ·L-1. 重点考察了乙腈和正己烷两种常用有机溶剂对 双酚A免疫传感分析的影响,初步探讨了有机溶剂对免疫传感分析的影响机制. 结果表明,正己烷含量高达 10%都不会对 双酚A免疫传感分析产生明显影响,而乙腈的影响比较大. 当乙腈含量较低时,可用于 双酚A的定量检测; 而当乙腈含量较高时,可完全抑制均相溶液中抗体抗原之间的亲和反应,不能用于 双酚A的定量分析. 其原因可能是乙腈取代了一部分结合在抗体表面的水分子,导致抗体构象改变或者抗体与抗原的亲和力变化而造成的. |
| 英文摘要 |
| Based on indirect competitive immunoassay mechanism, bisphenol A (BPA) was detected by the evanescent wave all-fiber immunosensor previously developed with the detection limit of 0.2 μg ·L-1 and the linear detection range of 0.3-33.4 μg ·L-1. The effects of two commonly used organic solvents, including acetonitrile and n-hexane, on the immunosensing assay of BPA were investigated. The influence mechanism of organic solvents on immunosensing assay was discussed. The experimental results showed that the effect of n-hexane on immunosensing assay was negligible even at a high concentration of up to 10%, whereas the effect of acetonitrile on the immunosensing assay was relatively great. BPA could be detected in solutions containing a low concentration of acetonitrile. However, the specific binding reaction between antibody and antigen in homogeneous solution was completely inhibited by high concentrations of acetonitrile, and the quantitative analysis of BPA was not achieved. This might result from the changes of antibody conformation or binding capability between antibody and antigen because acetonitrile replaced a part of the water molecules on the antibody surface. |
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