| 温度分化对APBR反应器性能及产甲烷菌群落的影响 |
| 摘要点击 2022 全文点击 1070 投稿时间：2014-05-22 修订日期：2014-11-28 |
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| 中文关键词 厌氧固定床反应器 (APBR) 温度 牛粪 产甲烷菌群落 16S rRNA基因 Q-PCR |
| 英文关键词 anaerobic packed bed reactor(APBR) temperature dairy manure methanogenic community 16S rRNA gene Q-PCR |
| 作者 | 单位 | E-mail | | 谢海迎 | 西南大学资源环境学院, 重庆市能源微生物重点实验室, 重庆 400716 | xhy702@163.com | | 汪鑫 | 西南大学资源环境学院, 重庆市能源微生物重点实验室, 重庆 400716 | | | 李牧原 | 西南大学资源环境学院, 重庆市能源微生物重点实验室, 重庆 400716 | | | 阎叙酉 | 西南大学资源环境学院, 重庆市能源微生物重点实验室, 重庆 400716 | | | 五十岚泰夫 | 西南大学资源环境学院, 重庆市能源微生物重点实验室, 重庆 400716 | | | 罗锋 | 西南大学资源环境学院, 重庆市能源微生物重点实验室, 重庆 400716 | van77@swu.edu.cn |
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| 中文摘要 |
| 为了研究温度分化对固定床厌氧反应器 (anaerobic packed bed reactor,APBR)牛粪发酵处理效果及产甲烷菌群落的影响,反应器发酵温度从室温 (22℃±1℃)阶梯式分化到低温 (15℃±1℃)、 中温 (37℃±1℃)和高温 (55℃±1℃). 温度变化的过程中,温度越高 COD(chemical oxygen demand)去除率和日总产气量越高, 分化后COD去除率分别为25%、 45%、 60%,相应的日产气量为2.3、 4.0、 8.5 L ·d-1,但是甲烷含量基本保持不变(~ 60%); 温度突然变化造成挥发性脂肪酸含量骤然增加,并处于波动状态. 16S rRNA基因克隆文库法分析表明,室温时包含广古菌门中的常见重要产甲烷菌 MBT(甲烷杆菌目)、 Mst(甲烷鬃菌科)、 Msc(甲烷八叠球菌科)和 MMB(甲烷微菌目),以及嗜热菌,也有少部分泉古生菌门,发酵温度分化后,产甲烷菌多样性减少,中温条件下产甲烷菌种类相对较少. 定量 PCR表明 Mst、 MMB和 Msc总基因浓度都有所减少,并且温度越高减少越多,各菌数量相对比例变化较大,但 Mst仍为优势产甲烷菌. |
| 英文摘要 |
| This study aimed to analyze the effect of temperature on performance and microbial community structure of an anaerobic packed bed reactor (APBR). The temperature was increased step-wise from room temperature (22℃±1℃) to psychrophilic (15℃±1℃), mesophilic (37℃±1℃) and thermophilic (55℃±1℃). The results showed that, in the temperature changing process, the higher the temperature of APBR was, the higher COD removal rate and daily gas production were. After temperature changed to psychrophilic, mesophilic and thermophilic, COD removal rate and daily gas production were 25%, 45%, 60% and 2.3 L ·d-1, 4.0 L ·d-1, 8.5 L ·d-1 respectively. However, there was no significant change in biogas composition (~60%). A sudden temperature change caused a simultaneous increase in the concentration of volatile fatty acids (VFA), which had been fluctuating. Using 16S rRNA gene clone library screening, Euryarchaeota was commonly found, including important methanogens: MBT (Methanobacteriales), Mst (Methanosaetaceae), Msc (Methanosarcinaceae) and MMB (Methanomicrobiales), as well as thermophilic bacteria and few spring Archaea. However, the diversity of methanogenic groups was reduced, especially at mesophilic. The results of quantitative PCR showed that the 16S rRNA gene concentrations of Mst, MMB and Msc were reduced by temperature changes. Although the relative proportion of every kind of methanogen was significantly affected, Mst was the dominant methanogen. |
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