| 纳米氧化锌对斑马鱼肝脏的毒性效应 |
| 摘要点击 2635 全文点击 1275 投稿时间：2015-04-04 修订日期：2015-05-29 |
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| 中文关键词 纳米氧化锌 斑马鱼 肝脏 氧化应激 毒性效应 |
| 英文关键词 zinc dioxide nanoparticles zebrafish liver oxidative stress toxicity effect |
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| 中文摘要 |
| 采用半静态急性实验方法,研究斑马鱼在浓度为0、0.05、0.1、5、10、25、50 mg·L-1的纳米氧化锌(nano-ZnO)中暴露4、24、96 h后,nano-ZnO对肝中超氧化物歧化酶(SOD)、丙二醛(MDA)、过氧化氢酶(CAT)的影响,肝脏中Bcl-2、Bax、p53和MDM2的mRNA表达量的变化以及暴露7、15、30 d时肝组织解剖结构变化情况. 结果表明,与对照组相比,实验组表现为: ①肝组织出现组织水肿,部分细胞质空泡化、细胞核固缩;②肝巨噬细胞增多,窦间隙增大;③肝中SOD活性升高、MDA含量增加、CAT活性降低;④肝脏中Bax/Bcl-2和p53的mRNA的表达量均升高;⑤ MDM2 mRNA的表达量表现为在低浓度组中降低,高浓度组中则升高. 实验结果表明,nano-ZnO能引起斑马鱼肝脏发生氧化应激作用,使肝中抗氧化酶活性发生变化,并诱导肝脏中细胞凋亡相关基因的表达,使细胞发生凋亡且造成肝组织结构发生变化. |
| 英文摘要 |
| For acute toxicity test, zebrafish were respectively exposed to the different concentrations of nano-ZnO (0, 0.05, 0.1, 5, 10, 25, 50 mg·L-1) for 4, 24 and 96 h.The superoxide dismutase(SOD), malondialdehyde(MDA), and catalase(CAT) in the liver of zebrafish were studied, and the relative expression of Bcl-2, Bax, p53 and MDM2 in liver were also determined.Anatomical structure of the zebrafish liver was determined after exposure to nano-ZnO for 7, 15 and 30 d.Compared with the control group, the liver of the experimental groups showed obvious difference in following aspects: ① oedema, cytoplastic vacuolation, and pyknotic nucleus were observed;② the number of hepatic macrophages deposited and the sinus clearance were increased;③ the MDA contents and the activity of SOD were increased;④ however, the activity of CAT was decreased;⑤ the mRNA expression level of genes Bax/Bcl-2 ratio and p53 of stressed groups were up-regulated;the mRNA expression level of gene MDM2 down-regulation can be observed in the low concentration groups while the mRNA expression level of gene MDM2 was up-regulated in the high concentration groups.The results suggested that, the oxidative damage of nano-ZnO to the zebrafish liver was caused by the increase of oxidative stress, which made the change of antioxidant enzyme activity, induced the expression of cell apoptosis genetic and cell apoptosis, and caused the change of organizational structure of liver. |
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