| 苯甲酸盐厌氧驯化体系中三氯乙烯的还原脱氯特性 |
| 摘要点击 1599 全文点击 938 投稿时间：2014-10-04 修订日期：2015-04-30 |
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| 中文关键词 三氯乙烯 454焦磷酸测序 实时荧光定量PCR 还原脱氯 脱卤拟球菌 |
| 英文关键词 trichloroethylene 454 pyrosequencing real-time quantitative PCR reductive dechlorinating Dehalococcoides spp. |
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| 中文摘要 |
| 采用气相色谱法实时监测了厌氧条件下,以苯甲酸盐为唯一碳源的驯化活性污泥体系对三氯乙烯(TCE)的还原脱氯情况;同时,结合454焦磷酸测序和实时荧光定量PCR技术对该体系中的微生物群落结构及脱卤拟球菌(DHC)的数量进行分析. 结果表明, 该驯化体系在94 d内能将TCE完全去除,最终转化产物为一氯乙烯(VC),同时伴随大量的甲烷产生;体系中微生物的多样性很高,涵盖了16个门、33个纲、52个目、88个科和129个属,而且体系中约有51.2%的微生物的分类地位尚未明确,说明体系中还存在很多未知的功能菌;体系对 TCE的降解过程其实就是还原脱氯菌与其它功能菌相互作用的结果,且起主要还原脱氯作用的是携带tceA功能基因的DHC. |
| 英文摘要 |
| Gas chromatography was used to monitor the reductive dechlorination of trichloroethylene (TCE) by anaerobic enrichment cultures with benzoate as the sole carbon source.The 454 pyrosequencing technique was used to investigate the microbial community and the real-time quantitative PCR was used to quantify the gene copies of Dehalococcoides spp.(DHC).The results showed that TCE was dechlorinated to vinyl chloride along with the formation of methane in 94 days.The anaerobic enrichment cultures exhibited a high diversity, which were classified into 16 phyla, 33 classes, 52 orders, 88 families and 129 genera, while 51.2% of them belonged to unclassified group, which inferred that there were a large portion of bacteria with unknown functional in this system.Degradation of TCE was accomplished by reductive dechlorinating and other functional populations, and the DHC which carried tceA gene could be the dominant reductive dechlorinating populations in the system. |
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