| 垃圾填埋场抗生素抗性基因初探 |
| 摘要点击 3002 全文点击 1350 投稿时间：2014-10-08 修订日期：2014-12-25 |
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| 中文关键词 垃圾填埋场 理化因子 荧光定量PCR 抗生素抗性基因 相关性分析 |
| 英文关键词 landfill physicochemical factors fluorescence quantitative PCR antibiotic resistance gene (ARGs) relevance analysis |
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| 中文摘要 |
| 不同环境介质中抗生素抗性基因普遍存在,但是在垃圾填埋场中抗生素抗性基因尚无相关报道. 本实验以西安江村沟垃圾填埋场为研究对象,采集不同方位不同深度垃圾样品,分析垃圾理化性质,用荧光定量PCR检测磺胺类抗生素抗性基因(sul Ⅰ 和sul Ⅱ )、抗氯霉素类抗生素抗性基因(cat)、β-内酰胺类抗生素抗性基因(bla-SHV),以及四环素类抗生素抗性基因(tetW)等5种抗生素抗性基因的含量,以相关性分析垃圾理化性质与抗性基因的关联. 结果表明,5种抗生素抗性基因均存在于垃圾中,基因拷贝数(以干土计)最大值分别为: (3.70±0.06)×108 copies ·g-1(sul Ⅱ )、(9.33±0.06)×106 copies ·g-1(sul Ⅰ )、(2.27±0.08)×105 copies ·g-1(tetW)、(3.68±0.09)×104 copies ·g-1(bla-SHV)和(1.39±0.10)×104 copies ·g-1(cat),说明垃圾填埋场是抗生素抗性基因潜在的储存库. 抗性基因sul Ⅰ、sul Ⅱ 和cat与含水率呈明显的正相关,同时sul Ⅰ 和cat基因含量与pH值呈负相关. |
| 英文摘要 |
| Antibiotic resistant genes (ARGs), an emerging contaminant, have been detected worldwide in various environments such as sediments and river. However, little is known about ARGs distribution in landfill. In this study, we investigated five ARGs ［sulfonamides resistant genes (sul Ⅰ and sul Ⅱ ), chloramphenicols resistant gene (cat), β-lactams resistant gene (bla-SHV), and tetracyclines resistant gene (tetW)］ in refuse samples collected from jiangcungou landfill (Xi'an, China) by real-time PCR. We then correlated the ARGs and physiochemical properties of refuse to examine the link between them. Results showed that all tested ARGs have been detected in all samples, suggesting that landfill served as ARGs reservoir. The highest copies numbers of sul Ⅱ , sul Ⅰ , tetW, bla-SHV, and cat were (3.70±0.06)×108 copies ·g-1(dry refuse), (9.33±0.06)×106 copies ·g-1(dry refuse), (2.27±0.08)×105 copies ·g-1(dry refuse), (3.68±0.09)×104 copies ·g-1(dry refuse), and (1.39±0.10)×104 copies ·g-1(dry refuse), respectively. Further, sul Ⅰ, sul Ⅱ , and cat positively correlated to moisture and sul Ⅰ and cat negatively correlated to pH. |
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