| 喹啉降解菌筛选及其对焦化废水强化处理 |
| 摘要点击 2173 全文点击 1113 投稿时间：2014-09-29 修订日期：2014-12-08 |
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| 中文关键词 食酸菌属 喹啉 降解性能 降解动力学 生物强化 |
| 英文关键词 Acidovorax sp. quinoline degradation properties degradation kinetics enhanced biotreatment |
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| 中文摘要 |
| 以喹啉为目标污染物,从焦化厂废水处理工段活性污泥中分离出1株能利用喹啉作为唯一碳源、氮源及能源的高效降解菌DQS-01,经16S rRNA基因分析鉴定为丛毛单胞菌科食酸菌属(Acidovorax sp.)菌株. 通过考察不同培养条件下DQS-01降解菌的生物量及喹啉降解率随时间的变化,确定其最佳降解条件为接种量10%、摇瓶转速150 r ·min-1、初始pH 8.0~10.0、温度35℃. 应用Haldane方程对该菌在不同喹啉初始浓度下的生长动力学过程进行了模拟,拟合曲线与实验测定值相关性良好. 将该菌与高效苯酚降解菌混合菌株用于焦化废水的生物强化处理,在移动床生物膜反应器(moving-bed biofilm reactor, MBBR)运行72 h后,对焦化废水COD的降解率达到87.4%. |
| 英文摘要 |
| A bacterial strain, which could utilize quinoline as the sole carbon, nitrogen and energy source, was isolated from the activated sludge in a coking wastewater treatment plant. According to the 16S rRNA gene sequence analysis, the strain was identified as Acidovorax sp. Taken into consideration of both the growth and the quinoline degradation of the strain, the optimized degradation conditions were acquired as following: 10% inoculum, pH value of 8.0-10.0, 35℃ and 150 r ·min-1. The process of its growth was simulated by Haldane kinetic model under different initial quinoline concentrations, the fitted curve had a good correlation with test measured values. Furthermore, coking wastewater was bioaugmented by the mixed strains of DQS-01 and D2 with enhanced process in a moving bed biofilm reactor, and the COD degradation rate was 87.4% within 72 h. |
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