| MBR处理腈纶废水的效能及微生物群落结构分析 |
| 摘要点击 2866 全文点击 1344 投稿时间：2014-04-26 修订日期：2014-07-03 |
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| 中文关键词 腈纶废水 膜生物反应器(MBR) 脱氮 微生物群落 PCR-DGGE |
| 英文关键词 acrylic fiber wastewater membrane bioreactor (MBR) denitrification microorganism community PCR-DGGE |
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| 中文摘要 |
| 采用序批式膜生物反应器（SBMBR）处理腈纶聚合废水和丙烯腈废水，考察了不同废水比例和运行条件下反应器的运行效果，并采用PCR-DGGE技术分析了反应器内微生物群落结构的变化.结果表明，SBMBR对腈纶废水具有较好的处理效果，碳源和碱度不足是影响脱氮效果的两个主要因素；在水力停留时间为24 h，90 min缺氧/150 min好氧交替运行的条件下，COD、NH4+-N和TN的平均去除率分别为82.5%、98.7%和74.6%，出水水质可以稳定达到国家污水综合排放标准（GB 8978-1996）的一级标准；PCR-DGGE分析结果表明，反应器内微生物多样性与进水水质和运行条件密切相关，微生物群落呈现出较明显的演替过程；通过克隆测序，从9个污泥样品中成功鉴定获得22种微生物的16S rDNA序列，并筛选出7种降解腈纶废水的优势菌种. |
| 英文摘要 |
| Sequencing batch membrane bioreactor (SBMBR) was used for the treatment of acrylic fiber polymerization wastewater and acrylonitrile wastewater. The operation efficiencies of SBMBR under different wastewater ratios and operation conditions were investigated, and the microbial community structure of the SBMBR system was analyzed by using PCR-DGGE technology. The results showed that SBMBR had a high removal efficiency on pollutants in acrylic fiber wastewater, and the lacking of carbon source and alkalinity were the main limiting factors for nitrogen removal. Under the designed operation conditions of 90 min anoxic/150 min aerobic cyclic operation and HRT of 24 h, the average COD, NH4+-N and TN removal efficiencies were 82.5%, 98.7% and 74.6%, respectively. The effluent of the SBMBR could steadily meet the Grade I standards of the Wastewater Comprehensive Discharge Standard of China (GB 8978-1996). The PCR-DGGE analyses showed that the microbial communities in SBMBR had a significant shift with the changes of influent characteristics and operation conditions. By cloning and sequencing analyses of selected dominant bacteria, 22 16S rDNA sequence were successfully identified from 9 sludge samples, from which 7 dominant functional microorganisms for the degradation of organic pollutants in acrylic fiber wastewater were screened out. |
