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饮用水处理中不同来源生物活性炭微生物群落多样性和结构研究
摘要点击 1537  全文点击 1080  投稿时间:2014-04-13  修订日期:2014-06-01
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中文关键词  饮用水  生物活性炭  微生物多样性  16S rRNA  RFLP
英文关键词  drinking water  biological activated carbon (BAC)  microbial diversity  16S rRNA  restriction fragment length polymorphism (RFLP)
作者单位E-mail
杜尔登 常州大学环境与安全工程学院, 常州 213164
同济大学污染控制与资源化研究国家重点实验室, 上海 200092 
duerdeng@cczu.edu.cn 
郑璐 常州市排水管理处, 常州 213162
同济大学污染控制与资源化研究国家重点实验室, 上海 200092 
 
冯欣欣 常州大学环境与安全工程学院, 常州 213164  
高乃云 同济大学污染控制与资源化研究国家重点实验室, 上海 200092 gaonaiyun@sina.com 
中文摘要
      通过限制性片段长度多态性技术考察了饮用水深度处理中5种不同来源生物活性炭的微生物群落多样性和结构. 单宁酸与腐殖酸吸附值相对较高的A炭、B炭和C炭的多样性指数较为接近,其微生物多样性更为丰富,而单宁酸与腐殖酸吸附值相对较低的D炭、E炭多样性指数较低. 生物活性炭样品的系统发育树中包含β-Proteobacteria、α-Proteobacteria、Planctomycetes、γ-Proteobacteria、Bacteroidetes等5类种群. 其中β-Proteobacteria和α-Proteobacteria是微生物群落的优势种群,对水中有机物的去除起到重要的作用. Planctomycetes、γ-Proteobacteria和Bacteroidetes是微生物群落的非优势种群. Bacteroidetes出现在A炭、B炭、C炭和D炭中,而没有出现在E炭中. 研究结果进一步加深了对生物活性炭中微生物群落的认识,为确保饮用水质安全提供理论基础.
英文摘要
      Restriction fragment length polymorphism (RFLP) technology was used to investigate the microbial diversity and structure of biological activated carbon (BAC) from different sources in drinking water advanced treatment process. Diversity indices of samples A, B and C, with relatively high tannic acid and humic acid adsorption capacity, were close to each other, which meant higher microbial diversity. However, samples D and E had relatively lower diversity indices with the low tannic acid and humic acid adsorption capacity. There were five species including β-Proteobacteria, α-Proteobacteria, Planctomycetes, γ-Proteobacteria, Bacteroidetes in the phylogenetic tree of BAC samples. Among them, β-Proteobacteria and α-Proteobacteria were the dominant microbial species in these BAC samples, which played an important role in organic matter removal. Planctomycetes, γ-Proteobacteria, and Bacteroidetes were the non-dominant microbial species. Bacteroidetes only existed in samples A, B, C and D, while did not occur in sample E. The BAC samples with the higher tannic acid and humic acid adsorption capacity had higher microbial diversity. This research should deepen the understanding of microbial community in BAC, and provide a theoretical basis for the safety of drinking water.

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