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温度对ABR-MBR复合工艺处理生活污水的影响及其微生物群落分析
摘要点击 2897  全文点击 1211  投稿时间:2014-01-09  修订日期:2014-04-08
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中文关键词  厌氧折流板反应器  膜生物反应器  生活污水  温度  种群分析
英文关键词  anaerobic baffled reactor  membrane bioreactor  domestic sewage  temperature  microbial analysis
作者单位E-mail
吴鹏 苏州科技学院环境科学与工程学院, 苏州 215009 wupengniu@126.com 
陆爽君 苏州科技学院环境科学与工程学院, 苏州 215009  
徐乐中 苏州科技学院环境科学与工程学院, 苏州 215009  
刘捷 苏州科技学院环境科学与工程学院, 苏州 215009  
沈耀良 苏州科技学院环境科学与工程学院, 苏州 215009
苏州科技学院江苏省环境科学与工程重点实验室, 苏州 215009 
ylshen@mail.usts.edu.cn 
中文摘要
      将厌氧折流板反应器(ABR)与膜生物反应器(MBR)优化组合(CAMBR)用于处理实际生活污水,研究温度对该反应器处理效能的影响,并采用变性梯度凝胶电泳(DGGE)技术对其内部微生物群落结构进行分析. 试验水力停留时间为7.5 h、混合液回流比R1为200%,R2为50%、pH为6.5~8.5、溶解氧3 mg ·L-1左右. 控制3个温度梯度:中温(25℃±5℃),低温(10℃±5℃),高温(35℃±5℃). 结果表明系统稳定运行后,温度对系统去除COD的影响很小,COD的去除效果很好. 中温和高温环境,系统出水水质较好,TN平均去除率为70%,出水平均浓度为9 mg ·L-1,TP平均去除率为73%,出水平均浓度低于0.8 mg ·L-1. 低温环境,TN平均去除率仅为57%,出水平均浓度为15 mg ·L-1;TP平均去除率降至67%,出水平均浓度为1 mg ·L-1. DGGE图谱表明,整个试验过程,系统内微生物类群保持多样性分布,同时优势菌群突出;在同一时期内,各反应池菌群相似性较高,但各隔室微环境的改变使得ABR和MBR内微生物菌群结构仍存在明显差异,强化了ABR和MBR的各自功能,有效保证了系统脱氮除磷效果.
英文摘要
      Effects of temperature on the combined process of ABR and MBR (CAMBR) for domestic sewage treatment were investigated and the changes in the bacterial community structure were analyzed by PCR-DGGE technique. The HRT, recycle ratio 1, recycle ratio 2, pH and DO were 7.5 h, 200%, 50%, 6.5~8.5 and 3 mg ·L-1, respectively. The temperature were controlled at three gradients: middle (25℃±5℃), low (10℃±5℃) and high (35℃±5℃). The results showed that the change of temperature had little influence on COD removal, and the CAMBR in stable state showed good performance in COD removal. In addition, the CAMBR achieved good effluent quality in middle or high temperature environment, and the average TN removal efficiency was 70% with an effluent TN of 9 mg ·L-1, and the average TP removal efficiency was 73% with the effluent TP below 0.8 mg ·L-1. For the process operated in low temperature environment, the average TN removal efficiency was only 57% with an effluent TN of 15 mg ·L-1, and the average TP removal efficiency was decreased to 67% with an effluent TP of 1 mg ·L-1. DGGE analysis indicated that throughout the process, the microbial population within the system maintained its diversity in distribution, while the dominant flora was prominent. During the same period, microbial populations in each compartment were similar. However, the structure of microbial community had significant differences between the ABR and the MBR due to the change of microenvironment in each compartment. Thus, the contributions of the ABR and the MBR were intensified, guaranteeing the efficiency of nitrogen and phosphorus removal in the system.

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