| 再生水中5种抗生素抗性菌的紫外线灭活及复活特性研究 |
| 摘要点击 2395 全文点击 1968 投稿时间：2013-08-08 修订日期：2013-09-23 |
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| 中文关键词 抗生素抗性菌 紫外线消毒 紫外线灭活 复活特性 再生水处理 |
| 英文关键词 antibiotic-resistant bacteria UV disinfection inactivation reactivation reclaimed water treatment |
| 作者 | 单位 | E-mail | | 黄晶晶 | 清华大学环境学院,环境模拟与污染控制国家联合重点实验室,北京 100084
中国电力工程顾问集团公司,北京 100120 | jjhuang@cpecc.net | | 汤芳 | 清华大学环境学院,环境模拟与污染控制国家联合重点实验室,北京 100084 | | | 席劲瑛 | 清华大学环境学院,环境模拟与污染控制国家联合重点实验室,北京 100084 | | | 庞宇辰 | 清华大学环境学院,环境模拟与污染控制国家联合重点实验室,北京 100084 | | | 胡洪营 | 清华大学环境学院,环境模拟与污染控制国家联合重点实验室,北京 100084
清华大学深圳研究生院,国家环境保护微生物利用与安全控制重点实验室,深圳 518055 | hyhu@tsinghua.edu.cn |
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| 中文摘要 |
| 抗生素抗性菌作为再生水中的新兴污染物而受到广泛关注.为探明紫外线对抗生素抗性菌的灭活和消毒后抗性菌的复活潜能，研究了以城市污水为水源的再生水（简称"再生水"）中青霉素抗性菌、氨苄青霉素抗性菌、头孢氨苄抗性菌、氯霉素抗性菌和利福平抗性菌的紫外线灭活特性，并考察了再生水中的抗生素抗性菌在黑暗条件下的复活潜能.结果表明，20 mJ·cm-2紫外线消毒剂量下，实际再生水中青霉素抗性菌、氨苄青霉素抗性菌、头孢氨苄抗性菌和氯霉素抗性菌的灭活率均高于4-log，与总异养菌群灭活率相当，而利福平抗性菌的灭活率（3.7-log）略低于总异养菌群.紫外线消毒后，再生水静置22 h后，抗生素抗性菌普遍出现复活现象，当紫外线消毒剂量为常规剂量20 mJ·cm-2时，消毒后再生水中的抗生素抗性菌菌落形成能力高达3-log.因此，常规的紫外线消毒剂量不能有效控制再生水储存或运输过程中抗生素抗性菌的复活. |
| 英文摘要 |
| Prevalence of antibiotic-resistant bacteria in wastewater effluents is concerned as an emerging contaminant. To estimate inactivation and reactivation potentials of antibiotic-resistant bacteria by UV disinfection, inactivation and reactivation of penicillin-, ampicillin-, cephalexin-, chloramphenicol-and rifampicin-resistant bacteria in the secondary effluent were studied under different UV doses. The results showed that the inactivation ratios of penicillin-, ampicillin-, cephalexin-and chloramphenicol-resistant bacteria were higher than 4-log, which was closed to that of total heterotrophic bacteria; however, the inactivation ratio of rifampicin-resistant bacteria was lower (3.7-log) under 20 mJ·cm-2 UV exposure. After 22 h standing incubation, antibiotic-resistant bacteria widely reactivated. The colony forming ability of antibiotic-resistant bacteria was as high as 3-log when exposed to 20 mJ·cm-2 UV light. Hence, conventional UV dose can not effectively control reactivation of antibiotic-resistant bacteria in reclaimed water by UV disinfection. |
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