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n(NO3--N)/n(NO2--N)对混培养菌与纯培养菌同步脱氮除硫的影响
摘要点击 2041  全文点击 1003  投稿时间:2013-04-07  修订日期:2013-07-08
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中文关键词  n(NO3--N)/n(NO2--N)  混培养  荧光假单胞菌  铜绿假单胞菌  脱氮  除硫
英文关键词  ratio of NO3--N to NO2--N  mixed culture  Pseudomonas fluorescens  Pseudomonas aeruginosa  nitrogen removal  sulfide removal
作者单位E-mail
陈子爱 农业部沼气科学研究所, 成都 610041 cza0903@163.com 
陈会娟 农业部沼气科学研究所, 成都 610041  
魏本平 农业部沼气科学研究所, 成都 610041  
刘刚金 农业部沼气科学研究所, 成都 610041  
邓良伟* 农业部沼气科学研究所, 成都 610041 dengliangwei@caas.cn 
吴力斌 农业部沼气科学研究所, 成都 610041  
中文摘要
      在温度30℃、pH为7、硫氮比为5/3、厌氧条件下,对比研究了不同n(NO3--N)/n(NO2--N)对荧光假单胞菌和铜绿假单胞菌混培养菌同步脱氮除硫影响. 随着n(NO3--N)/n(NO2--N)减小,荧光假单胞菌、铜绿假单胞菌对NOx--N去除率逐渐增高,而S2-去除率却依次减少,混培养菌对NOx--N去除效率先增加后趋于平稳. n(NO3--N)/n(NO2--N)对混培养菌去除S2-几乎没有影响. 荧光假单胞菌能迅速将NO3--N转化为NO2--N,但NO2--N转为N2却相对缓慢,培养液中出现NO2--N累积;而铜绿假单胞菌将NO2--N还原N2的能力明显比荧光假单胞菌强,培养液未反应的NOx--N以NO3--N为主,未出现NO2--N累积. 混培养菌对NOx--N转化的情况介于荧光假单胞菌与铜绿假单胞菌之间. 荧光假单胞菌同时获得较高NOx--N、S2-去除的n(NO3--N)/n(NO2--N)为5/5,铜绿假单胞菌为10/0,混培养菌为5.0/5.0. 混培养菌对NOx--N、S2-的同步去除效果优于单菌株荧光假单胞菌和铜绿假单胞菌.
英文摘要
      Effects of the ratio of NO3--N to NO2--N on removal of sulfide and nitrogen by Pseudomonas fluorescens, Pseudomonas aeruginosa and mixed culture were investigated at the pH value of 7.0, temperature of 30℃, ratio of sulfide to nitrate of 5/3 and anaerobic condition. Along with the decrease of ratio of NO3--N to NO2--N, NOx--N, the removal rate by Pseudomonas fluorescens and Pseudomonas aeruginosa increased gradually, while S2- removal rate reduced. The NOx--N removal rate by mixed culture increased first and became steady along with the decrease of ratio of NO3--N to NO2--N. The ratio of NO3--N to NO2--N had hardly influence on S2- removal by mixed culture. Accumulation of NO2--N occurred due to a faster rate of NO3--N reduction over NO2--N reduction in the liquid culture of Pseudomonas fluorescens. Accumulation of NO2--N did not occur in the liquid culture of Pseudomonas aeruginosa because it has a stronger ability to convent NO2--N than Pseudomonas fluorescens. The behavior of NOx--N by mixed culture in located between Pseudomonas fluorescens and Pseudomonas aeruginosa. The optimum ratio of NO3--N to NO2--N to achieve high removals of sulfide and nitrogen for Pseudomonas fluorescens and mixed culture were 5/5, 10/0 for Pseudomonas aeruginosa. The performance of sulfide and nitrogen removal by the mixed culture was higher than that by Pseudomonas fluorescens and Pseudomonas aeruginosa.

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