| 二氯甲烷降解菌Methylobacterium rhodesianum H13的分离鉴定及降解特性研究 |
| 摘要点击 2278 全文点击 1237 投稿时间：2012-12-02 修订日期：2013-01-08 |
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| 中文关键词 二氯甲烷 生物降解 甲基杆菌 分离鉴定 降解性能 |
| 英文关键词 dichloromethane biodegradation methylobacterium isolation and identification degradation characteristics |
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| 中文摘要 |
| 对分离筛选到1株能以二氯甲烷(DCM)为唯一碳源和能源生长的菌株Methylobacterium rhodesianum H13进行降解特性研究. 在初始菌体浓度0.82 mg ·L-1、pH 7.0和温度30℃的条件下, M. rhodesianum H13能够于23 h内将5 mmol ·L-1 DCM完全降解,细胞得率(细胞/DCM)为0.136 g ·g-1. 随着DCM的降解,溶液中的Cl-浓度逐渐升高(释放的Cl-浓度约为DCM的2倍),溶液pH值降至6.75,呈弱酸性. 通过摇瓶实验考察了温度、pH、DCM浓度、Cl-等因素对菌株H13降解DCM性能的影响,获得其较适宜的生长和降解条件为:温度30℃、pH值7.0. 研究还发现M. rhodesianum H13降解DCM的最适浓度为5 mmol ·L-1,高浓度的DCM会抑制其降解. 研究成果对高效处理环境中的DCM污染具有重要的应用价值. |
| 英文摘要 |
| A dichloromethane-degrading bacterium Methylobacterium rhodesianum H13 which utilized the DCM as the sole carbon and energy source was isolated. According to the research, M. rhodesianum H13 could completely degrade 5 mmol ·L-1 DCM in 23 h with the initial cell concentration of 0.82 mg ·L-1,pH 7.0, 30℃, and the cell yield rate was about 0.136 g ·g-1 DCM. With the degradation of DCM, Cl- concentration gradually raised (the release of Cl- concentration was about 2 times higher as the DCM), pH value dropped to 6.75,and the solution was weakly acidic. Temperature, pH, DCM concentration, Cl- concentration and other factors were investigated through the shake flask experiments, and the optimal conditions for DCM degradation were: temperature 30℃, pH 7.0. The study also indicated that 5 mmol ·L-1 of DCM was the optimum concentration for M. rhodesianum H13 and high levels of DCM could inhibit the degradation. The research has an important application value for the DCM environmental pollution. |
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