| 扑草净降解菌的分离、筛选与鉴定及降解特性初步研究 |
| 摘要点击 2835 全文点击 1739 投稿时间：2012-10-11 修订日期：2012-12-26 |
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| 中文关键词 扑草净 微生物降解 鉴定 苍白杆菌属 芽胞杆菌属 |
| 英文关键词 prometryne biodegradation identification Ochrobactrum Bacillus |
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| 中文摘要 |
| 从安徽农药厂废水处理系统的活性污泥中筛选扑草净(prometryne)降解菌. 通过富集驯化和选择性培养筛选纯化细菌,分离到2株细菌,分别命名为P-1、P-2. 根据表型特征、生理生化特性和16S rDNA序列同源性分析:P-1为G-,鉴定为苍白杆菌属(Ochrobactrum); P-2为G+,鉴定为芽胞杆菌属(Bacillus). GenBank登录号分别为HM004554和HM004555. 通过12 d液体降解实验,菌株P-1、P-2对40 mg·L-1扑草净降解率分别达到46.5%和65.4%. 该研究为扑草净的生物降解与污染土壤的生物修复提供了依据. |
| 英文摘要 |
| The purpose of this study was to develop some bacterial strains that could degrade prometryne effectively. Bacteria were screened and purified from sediments of a pesticide plant with the method of domestication with gradient concentrations. Two strains were isolated and named P-1 and P-2, respectively. According to their morphology, physiological, biochemical properties and the analysis of their 16S rRNA gene sequences, the strain P-1 is Gram negative and identified as Ochrobactrum; the strain P-2 is Gram positive and identified as Bacillus. GenBank Accession numbers are HM004554 and HM004555, respectively. Degradation rates of the strains P-1 and P-2 for prometryne concentration with 40 mg·L-1 were 46.5% and 65.4% after 12 days in the liquid culture experiment, respectively. This study provides an essential material and theoretical foundation for the remediation of prometryne contaminated soils. |
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