| 1株苯并［a］芘高效降解菌的筛选与降解特性 |
| 摘要点击 2900 全文点击 1057 投稿时间：2012-07-22 修订日期：2012-10-25 |
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| 中文关键词 苯并［a］芘 短短芽胞杆菌 筛选 生物降解 特性 |
| 英文关键词 benzo［a］pyrene Brevibacillus brevis screening biodegradation characteristics |
| 作者 | 单位 | E-mail | | 蔡瀚 | 暨南大学环境工程系,广东省高校毒害性污染物防治与生物修复重点实验室,广州 510632 | caihan1987416@sina.com | | 尹华 | 华南理工大学环境科学与工程学院,广州 510006
工业聚集区污染控制与生态修复教育部重点实验室,广州 510006 | thyin@jnu.edu.cn | | 叶锦韶 | 暨南大学环境工程系,广东省高校毒害性污染物防治与生物修复重点实验室,广州 510632 | | | 常晶晶 | 暨南大学环境工程系,广东省高校毒害性污染物防治与生物修复重点实验室,广州 510632 | | | 彭辉 | 暨南大学环境工程系,广东省高校毒害性污染物防治与生物修复重点实验室,广州 510632 | | | 张娜 | 暨南大学环境工程系,广东省高校毒害性污染物防治与生物修复重点实验室,广州 510632 | | | 何宝燕 | 暨南大学环境工程系,广东省高校毒害性污染物防治与生物修复重点实验室,广州 510632 | |
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| 中文摘要 |
| 从广东省贵屿镇受污染河涌底泥中筛选出1株能利用苯并［a］芘(BaP)作为唯一碳源和能源生长的高效降解菌株. 经生理生化试验和16S rRNA序列测定,鉴定该菌为短短芽胞杆菌(Brevibacillus brevis). 该菌在7 d内对1 mg·L-1 BaP的降解率达51.35%. 降解性能研究表明,pH、温度、投菌量、BaP初始浓度和处理时间等是影响BaP降解的重要因素. B. brevis对pH与温度均有较广泛的耐受范围,在pH 2~12和温度为25~40℃的范围内对BaP均有降解能力,中性(pH=7)和室温(25℃)为最优条件. 随着投菌量的增加,B. brevis对BaP的处理能力呈先增强后平缓的趋势; 而BaP初始浓度的增加,则使降解量呈不断上升的趋势. 水杨酸、琥珀酸和邻苯二甲酸的加入并不能明显提高B. brevis对BaP的降解效果. 处理BaP后菌体表面褶皱增多,随着时间的延长,菌体出现表面凹陷、瘪塌变形的现象. |
| 英文摘要 |
| A strain which could utilize BaP as a sole carbon and energy source and efficiently degrade benzo［a］pyrene (BaP) was isolated from the contaminated sediments of Guiyu, Guangdong province, China. The strain was identified as Brevibacillus brevis based on physiological and biochemical experiments together with 16S rRNA sequence analysis. The experimental results showed that the biodegradation rate of BaP by B. brevis in 7 days was 51.35%. The study also demonstrated that pH, temperature, bacterial dosage, initial concentration of BaP and processing time were important factors for BaP degradation. B. brevis could tolerate wide pH and temperature ranges, from 2 to 12℃ and 25 to 40℃, respectively. The optimum condition for BaP degradation was pH 7 and 25℃. With the increase of B. brevis inoculation amount, the degradation efficacy displayed an initial increasing trend and then came to a plateau. And the increase of BaP concentration led to the enhancement of BaP degradation. Addition of salicylic, succinate and phthalate showed no obvious positive effect on BaP biodegradation. After degradation of BaP, the surface of B. brevis was wrinkled, and became depressed and deformed over time. |
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