| 16S rDNA克隆文库分析高含盐生物脱硫系统细菌多样性 |
| 摘要点击 2099 全文点击 1503 投稿时间：2012-04-25 修订日期：2012-06-30 |
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| 中文关键词 生物脱硫 16S rDNA 无色硫细菌 高盐含硫废水 细菌多样性 |
| 英文关键词 biological desulfurization 16S rDNA colorless sulfur bacteria high salinity wastewater containing sulfide bacterial diversity |
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| 中文摘要 |
| 采用分子生物学手段16S rDNA克隆文库方法研究连续运行1 a的生物脱硫反应器中细菌的多样性.从16S rDNA克隆文库中随机挑选40个克隆子进行序列测定(约1400 bp),对测序结果进行了Blast对比.结果表明,脱硫系统中存在比例较高的优势菌种,有33个克隆子分属于3个不同的细菌类群,1个克隆子属于未知类群,优势细菌类群为Proteobacteria类群(变形菌类群),占85.3%.细菌类群优势顺序为:γ-Proteobacteria类群(55.9%),β-Proteobacteria类群(17.6%),Actinobacteridae类群(8.8%),δ-Proteobacteria(5.9%),α-Proteobacteria(5.9%),Sphingobacteria(2.9%).其中盐生硫杆菌属的Halothiobacillus sp. ST15和硫杆菌属的Thiobacillus sp. UAM-I是系统中的主要脱硫细菌. |
| 英文摘要 |
| The bacterial diversity in the biological desulfurization reactor operated continuously for 1 year was studied by the 16S rDNA cloning and sequencing method. Forty clones were randomly selected and their partial 16S rDNA genes (ca.1400 bp) were sequenced and blasted. The results indicated that there were dominant bacterias in the biological desulfurization reactor, where 33 clones belonged to 3 different published phyla, while 1 clone belonged to unknown phylum. The dominant bacterial community in the system was Proteobacteria, which accounted for 85.3%. The bacterial community succession was as follows: the γ-Proteobacteria(55.9%), β-Proteobacteria(17.6%), Actinobacteridae(8.8%), δ-Proteobacteria(5.9%), α-Proteobacteria(5.9%), and Sphingobacteria(2.9%). Halothiobacillus sp. ST15 and Thiobacillus sp. UAM-I were the major desulfurization strains. |
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