| 链霉菌FX645对偶氮染料红AR30的降解机制研究 |
| 摘要点击 1518 全文点击 862 投稿时间：2012-04-21 修订日期：2012-06-30 |
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| 中文关键词 偶氮染料 脱色降解 链霉菌 降解机制 偶氮还原酶 |
| 英文关键词 azo dye decolourisation and degradation Streptomyces sp. degradation mechanism azo reductase |
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| 中文摘要 |
| 从印染厂污泥中筛选到1株对偶氮染料红30(AR30)具有较强脱色降解作用的菌株Streptomyces sp. FX645.通过降解产物的紫外-可见吸收光谱、LC-MS分析及各降解产物在发酵体系中浓度随时间变化的规律,探讨了菌株FX645对AR30的可能降解途径,首先在偶氮还原酶的作用下AR30发生偶氮键的裂解,生成4-硝基-2,6-二氯苯胺与2-［(4-胺苯基)-(2-氰乙基)-氨基］乙酸乙酯,然后分别发生硝基还原、氨基酰化、氰基水解作用,生成一系列的苯胺类化合物. |
| 英文摘要 |
| One strain, identified as Streptomyces sp. FX645 which was isolated from the sludge collected in a printing and dyeing mill, had high potency of degradation and decolourisation of azo dye Red 30 (AR30). The microbial degradation mechanism on AR30 by strain FX645 was proposed through analyzing the UV-vis spectra and LC-MS spectra of the degradation products and investigating the variations in the concentrations of the degradation products in the culture. It is suggested that the azo bond of AR30 was initially cracked by azo reductase to produce 2,6-dichloro-4-nitrobenzenamine and 2-［(4-aminophenyl)-(2-cyanoethyl)amino］ethylacetate, which then generated several aromatic amine compounds under the actions of nitroreduction, aminoacylation and cyano hydrolysis, respectively. |
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