| 水稻秸秆浸泡液对铜绿微囊藻生理特性的影响 |
| 摘要点击 2293 全文点击 1377 投稿时间：2012-03-20 修订日期：2012-06-19 |
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| 中文关键词 秸秆 流式细胞仪 铜绿微囊藻 光合作用 酯酶活性 |
| 英文关键词 rice straw flow cytometry Microcystis aeruginosa photosynthesis esterase activity |
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| 中文摘要 |
| 室内利用流式细胞仪对暴露于不同浓度水稻秸秆浸泡液下铜绿微囊藻的细胞生长、细胞膜完整性、膜电位、酯酶活性进行了为期15 d的检测,研究了水稻秸秆浸泡液对铜绿微囊藻生理特性的影响. 结果发现,浸泡5 d的水稻秸秆液可以抑制藻的生长,呈明显的浓度抑制型变化; PI荧光检测显示暴露于浸泡液的各组细胞(>98%)的细胞膜保持高度完整; FDA荧光检测显示与对照组相比,第1、4 d处理组酯酶活性增强和减小的细胞都有增加,但活性下降的细胞数量明显多于活性增强的细胞,第7 d酯酶活性下降的细胞数量明显增加,而增强的细胞数量基本不变,第10、15 d酯酶活性正常的细胞数量增加显著,而酯酶活性下降的细胞数量明显减少,增强的细胞变化幅度较小; DIOC6(3)荧光检测显示膜电位在前7 d变化显著,第10、15 d变化程度减弱,与酯酶活性变化趋势一致. 分析表明,浸泡液存在促进和抑制藻细胞生长的两种作用,抑制作用占据优势,随着暴露时间延长,促进作用消失,抑制作用有所下滑,浸泡液对藻细胞生长具有抑制性而非致死性的作用. |
| 英文摘要 |
| The growth and physiology of bloom-forming cyanobacterium Microcystis aeruginosa were determined by the flow cytometry when exposed to rice straw extract for 15 d. The cell growth, cell integrity, mitochondrial transmembrane potential, and esterase activity were used to evaluate the physiological response in Microcystis aeruginosa. Rice straw extract stored for 5 days significantly inhibited the growth of Microcystis aeruginosa in a concentration-dependent way; Most of the algae cells (>98%) remained complete membranes in all the concentration treatments; Compared with the control cultures, the rice straw induced both negative and positive effects on the esterase activity for each test within 4 days, while the inhibition exceeded the stimulation effect. After a 7 d exposure, only the inhibition effect was found. Neither the inhibited nor stimulated effects was observed after algae exposure from 10 d to 15 d. Evident changes was found in the membrane potential during 7 d experiment, whereas inhibition effect became weaker after 10 d and 15 d exposure, in consistent with the result of esterase activity. These results confirmed that the rice straw extract might provide both dominant inhibition and relatively weak stimulation effects. After a long time exposure, inhibition effect became limited while stimulation effect disappeared. The action of rice straw may be algistatic (preventing algal growth) but not algicidal (killing algae). |
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