| 降解蒽嗜盐菌AD-3的筛选、降解特性及加氧酶基因的研究 |
| 摘要点击 3976 全文点击 1388 投稿时间：2012-02-16 修订日期：2012-04-09 |
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| 中文关键词 中度嗜盐菌 多环芳烃 蒽 生物降解 双加氧酶 |
| 英文关键词 moderately halophilic bacteria polycyclic aromatic hydrocarbons anthracene biodegradation dioxygenase |
| 作者 | 单位 | E-mail | | 崔长征 | 华东理工大学资源与环境工程学院,国家环境保护化工过程环境风险评价与控制重点实验室,上海 200237 | cuichangzheng@ecust.edu.cn, | | 冯天才 | 华东理工大学资源与环境工程学院,国家环境保护化工过程环境风险评价与控制重点实验室,上海 200237 | | | 于亚琦 | 国家知识产权局专利局专利审查协作北京中心,北京 100081 | | | 董婓 | 华东理工大学资源与环境工程学院,国家环境保护化工过程环境风险评价与控制重点实验室,上海 200237 | | | 杨昕梅 | 华东理工大学资源与环境工程学院,国家环境保护化工过程环境风险评价与控制重点实验室,上海 200237 | | | 冯耀宇 | 华东理工大学资源与环境工程学院,国家环境保护化工过程环境风险评价与控制重点实验室,上海 200237 | | | 刘勇弟 | 华东理工大学资源与环境工程学院,国家环境保护化工过程环境风险评价与控制重点实验室,上海 200237 | | | 林汉平 | 华东理工大学资源与环境工程学院,国家环境保护化工过程环境风险评价与控制重点实验室,上海 200237 | |
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| 中文摘要 |
| 蒽是典型的多环芳烃类环境污染物,属于美国EPA优先控制的16种多环芳烃类化合物,其在高盐环境下的生物降解备受关注. 本研究从某石油污染的高盐土壤中成功筛选出了1株高效降解蒽的菌株,经过对其生理生化特征和16S rDNA序列分析,初步鉴定并命名该菌株为Martelella sp. AD-3. 该菌株在0.1%~10%的盐度和6.0~10.0的pH范围内,均能够降解蒽. 其生长和降解蒽的优化条件是:蒽初始浓度25 mg·L-1、温度30℃、pH值9.0和盐度3%,在优化条件下培养6 d,蒽的降解率可达到94.6%. 根据已报道的双加氧酶α亚基的同源性设计简并引物,通过巢式PCR扩增获得双加氧酶基因的部分序列 307 bp (GenBank: JF823991.1),与海杆菌属Marinobacter sp. NCE312(AF295033)菌株萘双加氧酶大亚基的部分氨基酸序列同源性最高为95%. |
| 英文摘要 |
| Anthracene, among the 16 US EPA polycyclic aromatic hydrocarbons (PAHs), is a typical low molecular weight environmental contaminant, which gains concern on its biodegradation under hypersaline condition. In this study, an anthracene-degrading bacterial strain was isolated from highly saline petroleum-contaminated soil. Based on its physiological, biochemical characteristics and 16S rDNA sequence analysis, the bacteria was preliminary identified and named as Martelella sp. AD-3. The strain was able to utilize anthracene as sole carbon source for growth and the degradation occurred under broad salinities (0.1% to 10%) and varying pHs (6.0 to 10.0). The optimized degradation conditions were initial concentration 25 mg·L-1,culture temperature 30℃,pH 9.0 and salinity 3%. And 94.6% of anthracene was degraded by strain AD-3 under the optimal conditions within 6 days. Degenerate primers design was performed with a reported dioxygenase α subunit homologous gene. A length of 307 bp fragment of the partial dioxygenase gene sequences (GenBank accession: JF823991.1) was amplified by nested PCR. The clones amino acid sequence from strain AD-3 showed 95% identity to that of the partial naphthalene dioxygenase large-subunit from Marinobacter sp. NCE312 (AF295033). The results lay a foundation for the further study of molecular mechanism involved in the PAHs biodegradation by strain AD-3. |
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