| 1株1,2-二氯乙烷降解菌的分离及降解特性研究 |
| 摘要点击 2953 全文点击 1322 投稿时间：2011-12-18 修订日期：2012-03-12 |
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| 中文关键词 1,2-二氯乙烷 分离鉴定 降解特性 动力学 碳平衡 |
| 英文关键词 1,2-dichloroethane isolation and identification degradation characteristics kinetics carbon balance |
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| 中文摘要 |
| 从浙江某化工厂污水处理池的活性污泥中筛选分离到1株能以 1,2-二氯乙烷 (1,2-dichloroethane,1,2-DCA)为唯一碳源和能源生长的菌株 T-2,根据菌株的形态特征、生理生化特性及16S rRNA基因序列分析,该菌株被鉴定为 Starkeya sp. 研究表明,Starkeya sp. T-2生长和降解1,2-DCA较适宜的温度为30℃,较适宜的pH为 7.0~8.0; 菌株降解1,2-DCA的过程遵循Haldane动力学模型,其最大比生长速率μmax为0.065 h-1,最大比降解速率vmax为0.54 h-1; 菌株降解1,2-DCA的耐受浓度为500 mg·L-1,细胞产率系数(以1,2-DCA计)为0.191 mg·mg-1,菌株T-2利用1,2-DCA生长的过程中能同时把1,2-DCA最终矿化为CO2和H2O, 矿化率为45%. |
| 英文摘要 |
| A 1,2-dichloroethane-degrading bacterium T-2, which was able to utilize 1,2-dichloroethane as the sole carbon and energy source, was isolated from the activated sludge of a chemical plant in Zhejiang. According to the morphological, physiological and biochemical characteristics and sequence analysis of 16S rRNA, the strain was identified as Starkeya sp. T-2.The optimal temperature and pH for 1,2-DCA degradation were 30℃ and 7.0-8.0, respectively. The 1,2-DCA degrading process followed the Haldane kinetic model. The maximum specific growth rate and specific degradation rate were 0.065 h-1 and 0.54 h-1, respectively. The maximum tolerable concentration of 1,2-DCA for the strain was 500 mg·L-1, and the cell yield coefficient was 0.191 mg·mg-1. Strain T-2 could mineralize 1,2-DCA into CO2 and H2O, with a mineralization ratio of 45%. |
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