| 可可毛色二孢菌对焦化厂土壤多环芳烃污染修复 |
| 摘要点击 2939 全文点击 1662 投稿时间：2011-11-02 修订日期：2011-12-25 |
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| 中文关键词 可可毛色二孢菌 多环芳烃(PAHs) 修复 过氧化氢酶 转化酶 吐温80 羟丙基-β-环糊精 |
| 英文关键词 Lasiodiplodia theobromae polycyclic aromatic hydrocarbon (PAHs) remediation hydrogen peroxidase invertase Tween 80 hydroxypropyl-β-cyclodextrin(HPCD) |
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| 中文摘要 |
| 利用新型菌种可可毛色二孢菌(Lasiodiplodia theobromae)对北京焦化厂实际土壤PAHs污染进行修复,研究了该菌种在Tween 80和HPCD两种表面活性剂作用下对北京焦化厂土壤PAHs污染的强化修复效果,并探讨了修复过程中酶活动态变化及其与PAHs降解关系. 结果表明,在L. theobromae作用下,焦化厂土壤中PAHs降解率在第70 d达到45.3%,比控制组提高了30个百分点; 当Tween 80和HPCD在最佳含量(2 g ·kg-1和1 g ·kg-1)时,土壤中PAHs降解率达65.8%和63.9%,比控制组提高约50个百分点. 因此,研究证实L. theobromae是实际土壤PAHs污染修复的可选菌种,表面活性剂与L. theobromae联合修复实际土壤PAHs污染修复是一种可选技术. 在单一L. theobromae修复组和表面活性剂强化L. theobromae修复组中,土壤中过氧化氢酶和转化酶酶活性比控制组土壤中酶活性均提高2倍左右,表明L. theobromae可能产生过氧化氢酶和转化酶或者该菌种与土著微生物有协同作用. 相关性分析表明,过氧化氢酶和转化酶活性最大值与PAHs降解率相关系数分别是0.781和0.837, 转化酶活性与降解率的相关性高于过氧化氢酶. |
| 英文摘要 |
| Bioremediation of PAHs contaminated soil from Beijing Coking Plant was performed using a novel fungal strain Lasiodiplodia theobromae (L. theobromae). Moreover, enhanced bioremediation of PAHs contaminated soil was investigated in the presence of different concentrations of Tween 80 and hydroxypropyl-β-cyclodextrin (HPCD). The correlation of the dynamics of enzyme activities during remediation and the degradation of PAHs was analyzed. The results showed that the degradation rate of PAHs increased to 45.3% on the 70th day after addition of L. theobromae, which was 30 percentage points higher than that of the control group. At an optimum concentration of 2 g ·kg-1 for Tween 80 and 1 g ·kg-1 for HPCD, the degradation rate of PAHs was enhanced to 65.8% and 63.9%, respectively, which was 50 percentage points higher than that of the control group. Hydrogen peroxidase and invertase activities in soil in the bioremediation group with only L. theobromae and the surfactant enhanced group were both enhanced twice more than that of the control group. These results showed that L. theobromae may produce hydrogen peroxidase and invertase or have synergic effect with indigenous microorganisms. Correlation analysis showed that the correlation coefficients of PAHs degradation rate and maximum enzyme activities of hydrogen peroxidase and invertase were 0.781 and 0.837, respectively. Therefore, the correlation between invertase activities and degradation rate was higher. |
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