| 超声波促进好氧/缺氧污泥消化过程中水解酶活性变化研究 |
| 摘要点击 2359 全文点击 1082 投稿时间：2011-10-28 修订日期：2012-03-11 |
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| 中文关键词 超声波预处理 好氧/缺氧 污泥 淀粉酶 葡萄糖苷酶 蛋白酶 磷酸酶 |
| 英文关键词 ultrasonic pretreatment aerobic/anoxic sludge amylase glucosidase protease phosphatase |
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| 中文摘要 |
| 为评价超声波促进污泥好氧/缺氧消化的作用,采用频率28 kHz,声能密度为0.15 kW ·L-1和超声时间10 min的超声参数与好氧/缺氧消化衔接,研究了污泥的SS、VSS及水解酶活性(淀粉酶、α-葡萄糖苷酶、蛋白酶、磷酸酶)在消化过程中的变化趋势,同时以未经超声处理的消化污泥作为对照.结果表明,经过超声波-好氧/缺氧消化13 d,VSS去除率为44.3%,高出对照污泥14.9%.污泥中水解酶活性随消化天数增加呈先增加后减少的变化趋势,其中超声污泥的淀粉酶和葡萄糖苷酶活性在第5 d达到峰值,此时VSS淀粉酶活性为0.104 μmol ·g-1,葡萄糖苷酶活性为0.637 μmol ·g-1.超声污泥中胞内蛋白酶和胞外蛋白酶在第7 d达到峰值,胞内蛋白酶活性为23.68 μmol ·g-1,远远大于胞外蛋白酶,在污泥消化过程中起主导作用.超声污泥中酸性磷酸酶活性高于对照污泥,而碱性磷酸酶对环境改变较为敏感,超声波预处理改变污泥内部性质,导致碱性磷酸酶活性减少. |
| 英文摘要 |
| In order to evaluate the function of sludge aerobic/anoxic digestibility by ultrasonic pretreatment. The SS,VSS and hydrolytic enzyme activities(amylase, glucosidase, protease, phosphatase) were measured before and after ultrasonic pretreatment(28 kHz, 0.15 kW ·L-1, 10 min).The results showed that the performances of aerobic/anoxic were greatly improved after ultrasonic pretreatment, the removal efficiency of VSS went to 44.3%, 7.8% better than of traditional aerobic/anoxic digestion. The variational trend of sludge hydrolytic enzyme activities increased firstly and then fell off during 13d digestion, the maximum of amylase activity and glucosidase activity in ultrasonic sludge, appeared in the 5 d, amylase activity was 0.104 μmol ·g-1 and glucosidase activity was 0.637 μmol ·g-1. The maximum of intracellular protease activity and extracellular proteases activity in ultrasonic sludge, appeared in the 7 d, intracellular protease activity was 23.68 μmol ·g-1, higher than extracellular proteases activity, and it was playing a leading role in sludge digestion. The acid phosphatase activity of ultrasonic sludge was higher than the control sludge, and the alkaline phosphatase was sensitive to environment. So the alkaline phosphatase activity reduced when the internal properties of sludge was changed. |
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