| 曝气生物滤池工艺脱氮性能及反硝化细菌群落结构特征研究 |
| 摘要点击 3092 全文点击 1783 投稿时间：2011-10-10 修订日期：2011-11-24 |
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| 中文关键词 曝气生物滤池 前置反硝化脱氮工艺 C/N T-RFLP 反硝化细菌群落结构 |
| 英文关键词 biological aerated filter(BAF) pre-denitrification process C/N T-RFLP denitrification bacterial community structure |
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| 中文摘要 |
| 比较了两组不同填料组合的前置反硝化曝气生物滤池(biological aeration filter, BAF)脱氮工艺:陶粒-沸石BAF工艺(C-Z BAF)和沸石-陶粒BAF工艺(Z-C BAF),在不同C/N条件下的脱氮效果及滤料表面的反硝化细菌群落结构特征.结果表明,Z-C BAF对TN的平均去除效率高于C-Z BAF,且在低C/N条件下Z-C BAF的优势更加明显,当C/N为3.3时Z-C BAF的TN平均去除率比C-Z BAF高出20%.测定2种组合在不同C/N条件下的反硝化细菌群落结构发现,随着C/N的降低,2种组合反硝化细菌的物种丰度和多样性显著下降.低C/N条件下,Z-C BAF整个好氧滤池的nosZ基因优势T-RFs相对丰度>20%,而C-Z BAF好氧滤池顶端样品不能有效扩增到nosZ基因,可推断Z-C BAF的同步硝化反硝化功能优于C-Z BAF.在低C/N条件下Z-C BAF的脱氮效率明显高于C-Z BAF,因此Z-C BAF在低C/N且低浓度的有机废水处理方面更有潜力. |
| 英文摘要 |
| The nitrogen removal performance and the denitrification bacterial community structure of two pre-denitrification biological aeration filter (BAF) processes with different media combinations, the ceramic-zeolite BAF process (C-Z BAF) and the zeolite-ceramic BAF process (Z-C BAF), were compared at different C/N ratios. The results showed that the average TN removal efficiency of Z-C BAF was higher than that of C-Z BAF, and the advantage was more notable at low C/N ratio. When the C/N ratio decreased to 3.3, the average TN removal efficiency of Z-C BAF was 20% higher than that of C-Z BAF. Comparing the microbial community structure of the two combinations at different C/N ratios, it was found that the richness and diversity of denitrification bacterial community decreased significantly with the decrease of C/N ratio. At low C/N ratio, within the whole oxic filter of Z-C BAF, nosZ genetic T-RFs were detected with more than 20% relative abundance, whereas no nosZ genetic T-RFs were detected at the upper oxic filter of C-Z BAF by PCR amplification. The results indicated that the simultaneous nitrification and denitrification function of Z-C BAF was better than that of C-Z BAF. At low C/N ratio, the nitrogen removal efficiency of Z-C BAF was obviously higher than that of C-Z BAF. Thus, compared to C-Z BAF, Z-C BAF had greater potential on the treatment of wastewater with low organic concentration and low C/N ratio. |
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