| 蜜环菌漆酶对氯酚类污染物催化降解条件优化 |
| 摘要点击 2505 全文点击 1582 投稿时间：2011-09-23 修订日期：2011-11-15 |
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| 中文关键词 氯酚 漆酶 催化降解 蜜环菌 优化 |
| 英文关键词 chlorophenol laccase degradation Amillariella mellea optimization |
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| 中文摘要 |
| 利用蜜环菌发酵所得的漆酶粗酶液直接对2种氯酚类污染物2,4-二氯酚(2,4-DCP)和2-氯酚(2-CP)进行催化降解实验,探讨了反应时间、pH值、反应温度、氯酚浓度、以及漆酶酶量对其降解效果的影响,得出了最适降解条件并对其降解动力学进行分析.结果表明,在适宜的条件下,漆酶粗酶液可有效降解2,4-DCP和2-CP且蜜环菌漆酶催化降解2,4-DCP 的能力较强,2,4-DCP最适降解温度为40℃,最适浓度为75 mg·L-1,最适酶量为0.1 U·mL-1,最适pH值为6.5,在最优条件下反应10 h后,2,4-DCP最高降解率可达97%以上. 2-CP最适降解温度为50℃,最适浓度为100 mg·L-1,最适酶量为0.1 U·mL-1,最适pH值为6,在最优条件下反应10 h后,2-CP最高降解率高达93%以上. 蜜环菌漆酶粗酶液对2,4-DCP 和 2-CP的降解反应符合一级动力学特征.结果表明蜜环菌粗漆酶液能有效转化氯酚类化合物,说明该酶在酚类污染物治理和环境保护等方面有潜在应用价值. |
| 英文摘要 |
| Crude laccase extracted from the Amillariella mellea fermentation broth was directly used to catalyze the degradation of 2,4-chlorophenol (2,4-DCP) and 2-chlorophenol (2-CP). The effects of reaction time, pH, temperature, chlorophenol concentration, and laccase dosage on the removal efficiency of chlorophenols were investigated. Optimal catalytic conditions for the degradation of chlorophenols were obtained and the degradation kinetics were analyzed. The results indicated that the crude laccase from Amillariella mellea was able to effectively degrade 2,4-DCP and 2-CP, with higher catalytic ability towards 2,4-DCP degradation. For 2,4-DCP degradation, the optimal temperature was 40℃, the optimal substrate concentration was 75 mg·L-1, the optimal enzyme dosage was 0.1 U·mL-1, and the optimal pH was 6.5. Under these conditions, the maximum degradation rate of 2,4-DCP reached>97% after 10 h. For 2-DCP degradation, the optimal temperature was 50℃, the optimal substrate concentration was 100 mg·L-1, the optimal enzyme dosage was 0.1 U·mL-1, and the optimal pH was 6. Under these conditions, the maximum degradation rate of 2-CP was over 93% after 10 h. The reaction process of laccase-catalyzed 2,4-DCP and 2-CP degradation obeyed the first-order kinetics equation. The laccase from Amillariella mellea was able to effectively degrade chlorophenols, indicating its potential application value in phenolic pollutant control and environmental protection. |
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