| 多年蔬菜连作对土壤氨氧化微生物群落组成的影响 |
| 摘要点击 2550 全文点击 2003 投稿时间：2011-05-25 修订日期：2011-09-06 |
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| 中文关键词 蔬菜连作 硝化势 AOA和AOB群落组成 T-RFLP Q-PCR amoA基因 |
| 英文关键词 continuous cropping of vegetables nitrification potential(PNF) AOA and AOB community structure T-RFLP Q-PCR amoA gene |
| 作者 | 单位 | E-mail | | 孟德龙 | 中国科学院亚热带农业生态研究所亚热带农业生态过程重点实验室,长沙 410125
中国科学院桃源农业生态试验站,桃源 415700
中国科学院研究生院,北京 100049 | mengdelong1214@126.com | | 杨扬 | 中国科学院亚热带农业生态研究所亚热带农业生态过程重点实验室,长沙 410125
中国科学院桃源农业生态试验站,桃源 415700
中国科学院研究生院,北京 100049 | | | 伍延正 | 中国科学院亚热带农业生态研究所亚热带农业生态过程重点实验室,长沙 410125
中国科学院桃源农业生态试验站,桃源 415700
中国科学院研究生院,北京 100049 | | | 吴敏娜 | 中国科学院亚热带农业生态研究所亚热带农业生态过程重点实验室,长沙 410125
中国科学院桃源农业生态试验站,桃源 415700
新乡医学院,新乡 450003 | | | 秦红灵 | 中国科学院亚热带农业生态研究所亚热带农业生态过程重点实验室,长沙 410125
中国科学院桃源农业生态试验站,桃源 415700 | | | 朱亦君 | 中国科学院亚热带农业生态研究所亚热带农业生态过程重点实验室,长沙 410125
中国科学院桃源农业生态试验站,桃源 415700 | | | 魏文学 | 中国科学院亚热带农业生态研究所亚热带农业生态过程重点实验室,长沙 410125
中国科学院桃源农业生态试验站,桃源 415700 | wenxuewei@isa.ac.cn |
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| 中文摘要 |
| 为揭示农业生产中长期大量施用化学肥料对土壤硝化过程微生物种群的影响及其与土壤硝化能力的偶联关系,本研究通过在长沙黄兴蔬菜基地采集长期连作蔬菜(20 a以上,VL)和短期蔬菜种植地(2 a左右,VS)表层土壤(0~20 cm),利用末端限制性片段多态性(T-RFLP)和实时定量PCR(Q-PCR)等手段系统研究了蔬菜连作对氨氧化细菌(ammonia-oxidizing bacteria, AOB)和氨氧化古菌(ammonia-oxidizing archaea, AOA)的组成和丰度的影响及其与土壤硝化势的偶联关系.结果表明,长期蔬菜连作显著使得土壤中AOB amoA的组成趋于单一,同时也影响了土壤中AOA amoA的群落组成; RDA分析结果表明,影响AOB amoA群落结构的主要土壤因素为土壤pH以及Olsen-P的含量.土壤中AOA amoA基因拷贝数明显高于AOB,平均为细菌丰度的6倍,但土壤硝化势(PNF)与土壤中AOB amoA基因丰度成显著的正相关,而与土壤中AOA amoA基因丰度没有显著的相关性.尽管多年蔬菜连作对AOB和AOA丰度的影响还不清楚,却使得AOB优势种群富集,土壤硝化能力增强. |
| 英文摘要 |
| Investigations were conducted on the effects of intensive application of chemical fertilizers in crop production on soil nitrifier communities and the relationship between nitrifier communities and soil nitrification ability. Two series of vegetable soils were selected from Huangxing, Changsha, reflecting continuous vegetable cropping with about 20 years and new vegetable field with only about 2 years vegetable growing history. In each series five independent topsoils (0-20 cm) were sampled and each soil was a mixture of 10 cores randomly taken in the same field. Terminal restriction fragment length polymorphism (T-RFLP) and quantity PCR (Q-PCR) were used to determine the composition and abundance of ammonia-oxidizing bacteria (AOB) and ammonia-oxidizing archaea (AOA) communities. Results indicated that long-term and continuous vegetable cropping obviously changed the compositions of both AOB and AOA amoA gene, soil pH and Olsen-P content were the dominant factors affecting the composition of AOB amoA. In the vegetable soils, although the copy number of AOA amoA gene was about 5 times higher than AOB amoA gene, no significant correlation was detected between AOA amoA gene abundance and soil nitrification rate. It was not sure whether long-term and continuous vegetable cropping could shift the abundance of AOB and AOA, but it resulted in the enrichment of some dominant AOB species and increase of soil nitrification potential(PNF). |
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