| The removal capabilities and tolerance of high concentration of ammonia-nitrogen of heterotrophic nitrifiers were studied.Methods included multi-point sampling,domestication,gradient dilution of domestication liquid,color indicator as rapid nitrification detection and isolation from streaking plate were conducted to screen heterotrophic nitrifiers.The strains were identified according to the sequence analysis of 16S rDNA.After inoculating the strains into ammonia-nitrogen wastewater,changes of nitrogen compounds were measured in order to understand their denitrification characteristics.The denitrification efficiency was optimized by improving the C/N ratio,changing the compatibility of the strains and mixing the compatible strains with the domesticated bacterial suspension.Finally 8 high-efficiency heterotrohic nitrifiers were obtained,and named as N1-N8 respectively.Phylogenetic analysis showed that 8 strains belonged to Comamonas genus,Rhodococcusgenus,Pseudomonas genus,Arthrobacter genus and Paracoccus genus,respectively.When the initial concentration of ammonia nitrogen was 256.9 mg·L-1 and the C/N was 5.5 of the artificial wastewater,the removal rates of ammonia nitrogen by the strains were about 65%-80%,and the stain N4 was the best.When the C/N ratio of the wastewater increased to 8.0,the ammonia nitrogen removal rates of the strains correspondingly increased to about 80%-90%.As the strains compatibility,the denitrification rate of N4+N5+N6 was 88.2% in the artificial wastewater with initial ammonia nitrogen concentration was 261.1 mg·L-1 and initial C/N ratio was 5.5, which was higher than that of any single strain.The ammonia nitrogen removal rate could reach to 99.8% when N4+N5+N6 were combined with the domesticated bacterial suspension.In the artificial wastewater,when the initial ammonia nitrogen increased to 446.9 mg·L-1 and the C/N ratio decreased to 3.2,the ammonia nitrogen removal rate of the mixed strains which composed of N4+N5+N6 and domesticated bacterial suspension was 99.9%.There was almost no nitrite and nitrate nitrogen accumulated in eventually,and the total nitrogen removal rate was 66.5%.The nitrogen which was assimilated by the strains was only 33% of the lost ammonia nitrogen.It shows that the strains which could not be isolated in the domesticated bacterial suspension had significant synergies effects on ammonia nitrogen removal of the isolating strains. |
