| 污水处理厂二级出水中总异养菌群对6种抗生素的耐受性研究 |
| 摘要点击 3996 全文点击 3842 投稿时间：2011-01-21 修订日期：2011-05-30 |
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| 中文关键词 总异养菌数 抗生素抗性菌 耐受性 半抑制浓度 污水回用 |
| 英文关键词 total heterotrophic bacteria antibiotic-resistant bacteria antibiotic tolerance hemi-inhibitory concentrations of antibiotics wastewater reuse |
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| 中文摘要 |
| 以北京市2座污水处理厂二级出水为研究对象，通过考察总异养菌群、抗性菌比例、浓度及抗生素对细菌的半抑制浓度，研究了二级出水中一般细菌对青霉素、氨苄青霉素、头孢氨苄、氯霉素、四环素和利福平6种抗生素在不同浓度下的耐受性.结果表明， 2座污水处理厂出水中青霉素、氨苄青霉素、头孢氨苄和氯霉素抗性菌比例较四环素和利福平高.当抗生素浓度为32 mg·L-1时，污水处理厂G二级出水中头孢氨苄抗性菌比例最高为59%，而污水处理厂Q二级出水中氯霉素抗性菌比例最高为44%.头孢氨苄抗性菌在污水处理厂G、Q出水中的浓度分别高达4.0×103 CFU·mL-1和3.5×104 CFU·mL-1，而氯霉素抗性菌浓度分别高达4.9×102 CFU·mL-1和4.6×104 CFU·mL-1.污水处理厂G中异养菌对头孢氨苄的耐受能力最强，其半抑制浓度>32 mg·L-1；污水处理厂Q中，异养菌对氯霉素的耐受能力最强，其半抑制浓度为23.1 mg·L-1.污水处理厂二级出水中部分抗生素抗性菌污染严重，且稳定存在于低浓度抗生素的处理出水. |
| 英文摘要 |
| Prevalence of antibiotic-resistant bacteria in wastewater effluents is concerned as an emerging contaminant. To estimate antibiotic resistance in secondary effluents of municipal wastewater treatment plants, antibiotic tolerance of heterotrophic bacteria，proportion of antibiotic-resistant bacteria and hemi-inhibitory concentrations of six antibiotics (penicillin, ampicillin, cefalexin, chloramphenicol, tetracycline and rifampicin) were determined at two wastewater treatment plants(WWTPs)in Beijing. The results showed that proportions of ampicillin-resistant bacteria in WWTP-G and chloramphenicol-resistant bacteria in WWTP-Q were highest to 59% and 44%, respectively. The concentrations of ampicillin-resistant bacteria in the effluents of WWTP-G and WWTP-Q were as high as 4.0×103 CFU·mL-1 and 3.5×104 CFU·mL-1, respectively; the concentrations of chloramphenicol-resistant bacteria were 4.9×102 CFU·mL-1 and 4.6×104 CFU·mL-1, respectively. The data also indicated that the hemi-inhibitory concentrations of heterotrophic bacteria to 6 antibiotics were much higher than common concentrations of antibiotics in sewages, which suggested that antibiotic-resistant bacteria could exist over a long period in the effluents with low concentrations of antibiotics. Antibiotic-resistant bacteria could be a potential microbial risk during sewage effluent reuse or emission into environmental waters. |
