| Organphosphorus pesticides（OPs） can lead to serious environmental pollution problems. Whose bioeffect of multi-OPs is still lack of consideration although single component has been studied. To evaluate joint acute toxicity of OPs, two bioassay, AchE and T3 luminous bacterium, were studied, The result shows that: The sensitivity of AChE to different OPs are different: dichlorvos > trichlotfon > acephate > omethoate > triazophos, the detection limit of five kinds of OPs are 1.69, 3.78, 4.03, 7.32 and 8.03 μg/mL. Detecting the single toxicity of OPs with method of T3 luminous bacterium, the relationship between luminous efficiency of luminous bacterium and the concentration of OPs is negatively correlated, and the correlation coefficient is between 0.93 and 0.98, the EC50 value of OPs to T3 luminescent bacterium are 0.398, 0.712, 0.152, 0.094, 0.140 mg/mL, the toxicity order of OPs to T3 luminous bacterium is: acephate > triazophos > omethoate > dichlorvos > trichlorfon; Using such two kinds of methods to evaluate the joint toxicity of OPs showed that the joint mode action of binary mixtures of OPs includes the synergy effect and partial additive effect. |
