| 1株草除灵高效降解菌的分离鉴定与降解特性研究 |
| 摘要点击 2202 全文点击 1584 投稿时间：2010-06-23 修订日期：2010-08-23 |
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| 中文关键词 草除灵 分离鉴定 生长特性 菌体干重 生物降解 |
| 英文关键词 benazolin-ethyl isolation and identification growth characteristics cell dry weight biodegradation |
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| 中文摘要 |
| 从农药厂废水处理池的活性污泥中分离得到1株能以草除灵为唯一碳源生长的菌株.经生理生化鉴定和16S rRNA基因序列同源性分析，将此菌株初步鉴定为Methyloversatilis sp.，命名为MBLHC-2.对菌株MBLHC-2的生长特性研究表明，液体培养时，菌株呈絮状生长；在30℃、 pH 8.0的R2A培养基中生长最佳.菌株在20～40℃之间均能降解草除灵，在30℃、 pH 8.0的条件下菌株对草除灵的降解效率最高，48 h内降解率达99.78%.提高草除灵浓度会对菌株产生毒害作用；提高接种量有利于加快草除灵的降解，当接种量由0.5%提高到8%时，16 h时草除灵浓度由69.83 mg·L-1降低为1.41 mg·L-1；降解菌利用有机氮源降解草除灵效率最高；添加蛋白胨、酵母粉和LB溶液有利于菌株对草除灵的降解.草除灵终浓度为300 mg·L-1的工业废水经48 h处理后，草除灵的去除率达99.34%，菌株MBLHC-2具有很好的应用前景. |
| 英文摘要 |
| A highly efficient benazolin-ethyl degrading strain was isolated from pesticide wastewater treatment pool.This strain could use benazolin-ethyl as the sole carbon source for growth.It was identified as Methyloversatilis sp.according to its physiological and biochemical properties,and 16S rRNA gene sequence analysis.The strain was designated as MBLHC-2.The results of growth characteristics showed that cells of stain MBLHC-2 tended to clump together in liquid cultures; Stain MBLHC-2 grew best in R2A culture medium at 30℃ and pH 8.0.The strain could degrade benazolin-ethyl when temperature in the range of 20-40℃.The optimal temperature and pH was 30℃ and 8.0,respectively; The efficiency of benazolin-ethyl biodegradation in 48 h was 99.78%.Increasing concentration of benazolin-ethyl produced toxic effects on strain MBLHC-2; When the inoculation rate was 0.5% up to 8%,the residual concentration of benazolin-ethyl was 69.83 mg·L-1 down to 1.41 mg·L-1 in 16 h,which means that improving inoculation rate could speed up the degradation of benazolin-ethyl.Strain MBLHC-2 could degrade benazolin-ethyl more effectively in the use of organic nitrogen.Adding peptone,yeast extract and LB solution in the culture was beneficial to the degradation of benazolin-ethyl.Strain MBLHC-2 was capable of removing 300 mg·L-1 benazolin-ethyl in industrial wastewater by 99.34% in 48 h,and this strain possesses a good application potential for treatment of wastewater containing benazolin-ethyl. |
