| 尾矿废水对河流沉积物和稻田土壤细菌多样性的影响 |
| 摘要点击 2796 全文点击 2262 投稿时间：2010-06-29 修订日期：2010-09-01 |
| 查看HTML全文
查看全文 查看/发表评论 下载PDF阅读器 |
| 中文关键词 尾矿废水 重金属 16S rDNA 细菌多样性 PCR-DGGE |
| 英文关键词 tailings drainage heavy metals 16S rDNA microbial diversity PCR-DGGE |
|
| 中文摘要 |
| 采用PCR-DGGE以及分子克隆技术，探讨富含重金属的尾矿废水污染条件下稻田土壤以及河流沉积物中细菌群落结构和多样性变化情况.结果表明，铜尾矿库废水导致了河流沉积物(H1～H6)和稻田土壤(D5)的Fe、As、Cu、Pb和Zn污染，其含量远高于未受铜尾矿废水污染的稻田土壤(D1～D4)，且As、Fe、Cu的含量显著影响细菌群落结构.河流沉积物中细菌多样性低于稻田土壤，污染稻田土壤(D5)中细菌多样性高于未受污染的稻田土壤(D1～D4).DGGE优势条带的测序及比对结果显示河流沉积物和稻田土壤中细菌主要优势类群包括Actinobacteria、Acidobacteria、Firmicutes、α-Proteobacteria和β-Proteobacteria，但α-Proteobacteria在河流沉积物中不占优势. |
| 英文摘要 |
| The changes of structure and diversity of microbial communities in paddy soil and river sediment defiled by heavy metals were explored with PCR-DGGE and cloning technique. The results showed that paddy soil and river sediment were contaminated by drainage from the impoundment of copper mine tailings containing Fe,As,Cu,Pb and Zn.The contents of heavy metals in contaminated sediments (H1-H6) and paddy soil (D5) were much higher than those in the paddy soils (D1-D4).The As,Fe and Cu contents significantly affected the structure of bacterial community.The diversities of microbial communities in river sediments were lower than that in paddy soils.The contaminated paddy soil (D5) presented higher microbial diversity comparing to the uncontaminated paddy soils (D1-D4).The sequencing of prominent DGGE bands and BLAST results indicated that dominant taxa in river sediments and paddy soils were Actinobacteria,Acidobacteria,Firmicutes,α-Proteobacteria and β-Proteobacteria,whereas the α-Proteobacteria was not dominant in river sediments. |
