| 紫外线对铜绿微囊藻的抑制效果及特性研究 |
| 摘要点击 2542 全文点击 2366 投稿时间：2010-07-06 修订日期：2010-09-08 |
| 查看HTML全文
查看全文 查看/发表评论 下载PDF阅读器 |
| 中文关键词 紫外线 抑制 铜绿微囊藻 叶绿素 藻胆蛋白 |
| 英文关键词 UV inhibition Microcystis aeruginosa chlorophyll phycobilisome |
|
| 中文摘要 |
| 采用紫外平行光仪辐照方法，研究紫外线对“水华”蓝藻中常见的铜绿微囊藻生长的抑制效果，并探讨紫外线对藻沉降性能、叶绿素、藻胆蛋白的影响，以及藻生长光照条件对紫外线抑藻效果的影响.结果表明，紫外线对铜绿微囊藻的生长具有显著抑制作用，150 mJ/cm2紫外剂量下，藻细胞个数被控制于初始水平，藻生长受抑制，如增加紫外剂量，藻细胞个数逐渐降低，藻渐渐衰亡.紫外线辐照加快藻细胞沉降速率，初始浓度4.52×106个/mL的藻样经500 mJ/cm2紫外线照射，静置2 d后的沉降率为14.4%，高于对照样（1.5%）.叶绿素与藻胆体在经受紫外线辐照后均受破坏.150 mJ/cm2和300 mJ/cm2紫外辐照藻样并培养9 d后，叶绿素含量分别下降为原来的43.6%和26.4%；藻胆蛋白特征吸收峰下降明显.经紫外线照射后，优先进入黑暗环境有利于抑制藻复活，提高抑藻效果. |
| 英文摘要 |
| The inhibitory effects of ultraviolet (UV) irradiation on the growth of Microcystis aeruginosa was investigated, using the UV collimated beam apparatus. The experimental results indicated that, UV light had a high inhibitory effect on the growth of Microcystis aeruginosa. With UV dose of 150 mJ/cm2, the number of algal cells was controlled at the initial level. Under higher UV dose irradiation, the number of algal cells decreased greatly. Through UV irradiation treatment, sedimentation rate of algae was speeded. Under the UV irradiation of 500 mJ/cm2, the sedimentation rate of algae with initial concentration of 4.52×106 cells/mL increased to 14.4% from 1.5% after 2 days incubation. Chlorophyll and phycobilisome were destroyed to a certain extent after UV irradiation. With the UV irradiation of 150 mJ/cm2 and 300 mJ/cm2, the concentration of chlorophyll decreased to 43.6% and 26.4%, respectively. The characteristic absorption peak of phycobilisome was lowered obviously. After UV irradiation treatment, culturing the algae in the darkness first can maintain the inhibitory effect. |
